THE PHYSICAL MAPPING OF MICROSATELLITE MARKERS IN WHEAT
THE PHYSICAL MAPPING OF MICROSATELLITE MARKERS IN WHEAT
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DOI：
10.1139/g98-009
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发表时间：
1998-04
期刊：
影响因子：
3.1
通讯作者：
M. Röder;V. Korzun;B. Gill;M. Ganal
中科院分区：
文献类型：
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作者：
M. Röder;V. Korzun;B. Gill;M. Ganal
微卫星标记是植物中一类新的遗传标记。这样的标记揭示了高水平的多态性，即使在物种与一个狭窄的遗传基础，如六倍体小麦（小麦L.）。我们利用大量的微卫星标记和25个“中国春”的缺失群体进行了缺失定位实验，研究了二核苷酸微卫星标记在六倍体小麦同源异型群2染色体上的物理分布。31个微卫星标记在2A染色体上检测到14个微卫星位点，在2B染色体上检测到9个微卫星位点，在2D染色体上检测到10个微卫星位点。微卫星位点均匀分布沿着染色体长度，标记18 27个定义的物理间隔，包括着丝粒，间质和端粒区域。表观随机分布表明，微卫星标记提供了良好的小麦基因组覆盖率。
Microsatellite markers represent a new class of genetic markers in plants. Such markers reveal a high level of polymorphism even in species with a narrow genetic base, such as hexaploid wheat (Triticum aestivum L.). We used a large set of such markers and 25 deletion stocks of 'Chinese Spring' in a deletion-mapping experiment to study the physical distribution of dinucleotide microsatellite markers in homoeologous group 2 chromosomes of hexaploid wheat. Thirty-one microsatellite markers identified 14 loci in chromosome 2A, 9 loci in chromosome 2B, and 10 loci in chromosome 2D. The microsatellite loci were evenly distributed along the chromosome length, marking 18 of 27 defined physical intervals, including centromeric, interstitial, and telomeric regions. The apparent random distribution indicates that microsatellite markers provide excellent coverage of the wheat genome.Key words: deletion stocks, group 2 homoeologues, microsatellites, wheat.