Interference of Skp2 effectively inhibits the development and metastasis of colon carcinoma
Interference of Skp2 effectively inhibits the development and metastasis of colon carcinoma
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DOI：
10.3892/mmr.2014.2308
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发表时间：
2014-08-01
影响因子：
3.4
通讯作者：
Gao, Liping
中科院分区：
文献类型：
--
作者：
Chen, Haijin;Mo, Xiaodong;Gao, Liping
结肠癌是消化系统中常见的恶性肿瘤类型。本研究的目的是研究S期激酶相关的蛋白2（SKP2）在结肠癌中的作用，并确定SKP2-RNA干扰（RNAI）是否消耗SKP2是否会减轻结肠癌的增殖和迁移。基于SKP2基因序列设计了三对小干扰（Si）RNA，最有效的序列被用来沉默SW620细胞中的SKP2基因。在干扰后，使用定量聚合酶链反应和蛋白质印迹分析分别检测SKP-2 mRNA和蛋白质的表达。数据表明，SKP2-SIRNA有效抑制了增殖（P
Colon cancer is a common type of malignancy in the digestive system. The aim of the present study was to investigate the role of S-phase kinase-associated protein 2 (Skp2) in colon carcinoma and to identify whether depletion of Skp2 by Skp2-RNA interference (RNAi) attenuates the proliferation and migration of colon carcinoma. Three pairs of small interfering (si)RNA were designed based on the Skp2 gene sequence and the most effective one was used to silence the Skp2 gene in SW620 cells. Subsequent to the interference, quantitative polymerase chain reaction and western blot analysis were used for detecting the expression of Skp-2 mRNA and protein, respectively. The data demonstrated that the Skp2-siRNA effectively inhibited proliferation (P