The concept of drug dose for in vitro studies with chemotherapeutic agents.
The concept of drug dose for in vitro studies with chemotherapeutic agents.
复制标题
化疗药物体外研究的药物剂量概念。
作者：
K. Wheeler;V. Levin;D. Deen
在体外辐照过程中，组织培养细胞所受或所受的辐射剂量的测定，相对于化学试剂处理时这些细胞所受或所受药物剂量的测定而言，是比较直接的。在确定了药物暴露剂量和药物吸收剂量后，本文利用BCNU和CCNU培养大鼠9L脑肿瘤细胞的药代动力学和细胞存活数据，说明了在此类实验中充分评估适当药物剂量所涉及的问题和原则。这些实验问题的实际解决方案提出了许多这些情况下，药代动力学数据要么无法获得或不可用。希望这里说明的概念将为进行可解释的体外药物实验提供更统一的框架。
Determination of the radiation dose delivered to or received by tissue culture cells during irradiation in vitro is relatively straightforward compared to determination of the drug dose delivered to or received by these same cells when treated with chemicals agents. After defining drug exposure dose and drug absorbed dose, this paper uses pharmacokinetic and cell survival data collected from experiments where rat 9L brain tumor cells were treated in culture with BCNU and CCNU to illustrate the problems and principles involved in adequately assessing the appropriate drug dose in such experiments. Practical solutions to these experimental problems are presented for many of those situations where pharmacokinetic data is either unobtainable or unavailable. It is hoped that the concepts illustrated here will provide a more uniform framework for performing interpretable in vitro drug experiments.