BMP growth factors and Phox2 transcription factors can induce synaptotagmin I and neurexin I during sympathetic neuron development
BMP growth factors and Phox2 transcription factors can induce synaptotagmin I and neurexin I during sympathetic neuron development
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DOI：
10.1016/s0925-4773(01)00503-2
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发表时间：
2001-10-01
影响因子：
2.6
通讯作者：
Ernsberger, U
中科院分区：
文献类型：
--
作者：
Patzke, H;Reissmann, E;Ernsberger, U
编码参与神经递质分泌的蛋白质的突触结合蛋白I和neurexin I mRNA在去甲肾上腺素能递质表型的初始诱导后不久在初级交感神经节中变得可检测。为了测试这些更一般的神经元基因的诱导是否介导的信号，已知启动去甲肾上腺素能分化的神经元亚群，我们研究了它们的表达在去甲肾上腺素能神经元诱导异位过度表达的生长和转录因子。BMP 4或Phox2a在体内过表达导致突触结合蛋白I和neurexin I在异位定位的去甲肾上腺素能细胞中表达。在体外，除了酪氨酸羟化酶诱导外，BMP 4还启动突触结合蛋白I和neurexin I的表达。因此，突触结合蛋白I和neurexin I的诱导，这是在大量不同的神经元群体中表达，可以完成的生长和转录因子，只有一个子集的神经元。这些发现表明，参与神经递质分泌的蛋白质的初始表达在不同的神经元群体中受到不同信号的调节。(C)2001爱思唯尔科学爱尔兰有限公司保留所有权利。
Synaptotagmin I and neurexin I mRNAs, coding for proteins involved in neurotransmitter secretion, become detectable in primary sympathetic ganglia shortly after initial induction of the noradrenergic transmitter phenotype. To test whether the induction of these more general neuronal genes is mediated by signals known to initiate noradrenergic differentiation in a neuronal subpopulation, we examined their expression in noradrenergic neurons induced by ectopic overexpression of growth and transcription factors. Overexpression of BMP4 or Phox2a in vivo results in synaptotagmin I and neurexin I expression in ectopically located noradrenergic cells. In vitro, BMP4 initiates synaptotagmin I and neurexin I expression in addition to tyrosine hydroxylase induction. Thus, the induction of synaptotagmin I and neurexin I, which are expressed in a large number of different neuron populations, can be accomplished by growth and transcription factors available only to a subset of neurons. These findings suggest that the initial expression of proteins involved in neurotransmitter secretion is regulated by different signals in different neuron populations. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.