Granulocyte-macrophage colony-stimulating factor synergizes with interleukin-6 in supporting the proliferation of human myeloma cells [see comments]
Granulocyte-macrophage colony-stimulating factor synergizes with interleukin-6 in supporting the proliferation of human myeloma cells [see comments]
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粒细胞巨噬细胞集落刺激因子与白细胞介素 6 协同作用，支持人骨髓瘤细胞的增殖 [参见评论]
DOI：
10.1182/blood.v76.12.2599.2599
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发表时间：
1990
期刊：
影响因子：
20.3
通讯作者：
B. Klein
中科院分区：
文献类型：
--
作者：
Xinxin Zhang;R. Bataille;M. Jourdan;S. Saeland;J. Banchereau;P. Mannoni;B. Klein
The role of granulocyte-macrophage colony-stimulating factor (GM-CSF) in the growth of multiple myeloma (MM) was investigated in 21 patients with MM. In 17 patients with proliferating myeloma cells in vivo, recombinant GM-CSF significantly increased the endogenous-IL-6-mediated spontaneous myeloma cell proliferation occurring in 5-day cultures of tumor cells in vitro (P less than .01). Furthermore, GM-CSF was detected in 5-day culture supernatants of myeloma bone marrow cells. This endogenous GM-CSF was produced by the myeloma bone marrow microenvironment but not by myeloma cells and contributed to the spontaneous myeloma-cell proliferation observed in 5-day cultures. In fact, this proliferation was partially blocked (67%) by anti-GM-CSF monoclonal antibodies. The stimulatory effect of rGM-CSF was mediated through IL-6 because it was abrogated by anti-IL-6 monoclonal antibodies. rGM-CSF did not reproducibly increase the endogenous IL-6 production in short-term cultures of bone marrow cells of MM patients. Using an IL-6-dependent myeloma cell line (XG-1 cell line), rGM-CSF was shown to act directly on myeloma cells stimulating by twofold their IL- 6 responsiveness. rGM-CSF did not induce any IL-6 production in XG-1 cells, nor was it able to sustain their growth alone. Although no detectable GM-CSF levels were found in the peripheral or bone marrow blood of MM patients, it is possible that GM-CSF, produced locally by the tumoral environment, enhances the IL-6 responsiveness of myeloma cells in vivo in a way similar to that reported here in vitro.
干细胞增殖的协同因子：进一步研究靶干细胞以及白细胞介素1、白细胞介素6、粒细胞集落刺激因子的刺激机制。
影响因子：
20.3
作者：
Ikebuchi,K;Ihle,JN;Hirai,Y;Wong,GG;Clark,SC;Ogawa,M
通讯作者：
Ogawa,M
影响因子：
15.9
作者：
XU, WD;FIRESTEIN, GS;ZVAIFLER, NJ
通讯作者：
ZVAIFLER, NJ