The relation between Escherichia coli surface functional groups' composition and their physicochemical properties.

The relation between Escherichia coli surface functional groups' composition and their physicochemical properties.
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DOI:
10.1590/s1517-83822008000100003
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发表时间:
2008-01
期刊:
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
影响因子:
--
通讯作者:
Ellouali M
Ellouali M
中科院分区:
其他
文献类型:
--
作者:
Hamadi F;Latrache H;Zahir H;Elghmari A;Timinouni M;Ellouali M

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研究了大肠杆菌的表面特性,包括疏水性、电泳迁移率和表面官能团的组成。这些特征分别通过水接触角测量、微电泳和x射线光电子能谱(XPS)测定。考察了其理化性质与官能团组成的关系。pH值为7时,细胞表面的电泳迁移率似乎由(O=C)官能团控制。细胞表面的亲水性与高水平的(C-(O.N))和(OH- (C- o -C))官能团有关,而细胞表面的疏水性与(C-(C,H))官能团有关。
Escherichia coli surface characteristics including hydrophobicity, electrophoretic mobility and surface functional groups’ composition were investigated. These characteristics were determined respectively by water contact angle measurements, microelectrophoresis and x-ray photoelectron spectroscopy (XPS). The relation between the physicochemical properties and functional groups’ composition was also examined. The electrophoretic mobility at pH 7 appeared to be governed on the cell surface by the (O=C) functional groups. The cell surface’s hydrophilicity was associated with high levels of (C-(O.N)) and (OH- (C-O-C)) functional groups, whereas the cell surface’s hydrophobicity was associated with (C-(C,H)) functional groups.