Track of fate and primary metabolism of trifloxystrobin in rice paddy ecosystem
Track of fate and primary metabolism of trifloxystrobin in rice paddy ecosystem
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DOI：
10.1016/j.scitotenv.2015.03.028
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发表时间：
2015-06-15
影响因子：
9.8
通讯作者：
Wang, Mengcen
中科院分区：
文献类型：
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作者：
Cao, Mengchao;Li, Shuying;Wang, Mengcen
肟菌酯是一种甲氧基丙烯酸酯类杀菌剂，已广泛应用于防治多种作物特别是水稻的真菌病害。然而，其在稻田中的残留情况与其生态风险高度相关，但人们仍然对其知之甚少。为了阐明肟菌酯在稻田中的归宿和初级代谢，利用 DisQuE 提取试剂盒结合 GC-mu ECD 和 GC-EI-MS/MS 分析，开发了一种简单有效的分析方法。结果表明，肟菌酯在稻田水、土壤和稻草中添加量为0.005～2 mg·kg(-1)（对于水为mg·L-1）的方法学回收率为87.6%～109.1%，相对标准偏差(RSD)为1.9%～9.5%(n=5)，检出限(LOD、信噪比(S/N)=3)和定量限（LOQ，S/N=10）分别为6.3×10(-4)mg L-1和2.09×10(-3)mg L-1，表明该方法对稻田中痕量肟菌酯残留的有效监测具有良好的准确度、精密度和灵敏度。此外，残留肟菌酯的消散符合一级速率方程，显示半衰期为0.7至7.5天，说明肟菌酯在稻田中的降解速度普遍较快。此外，通过GC-EI-MS/MS分析确定，肟菌酯在稻田中的主要代谢物肟菌酯酸主要在稻田水中积累，并在14天内维持在2.41 mg L-1，这表明长期频繁使用该杀菌剂可能通过稻田排水对周围水生态系统的水生生物造成较高风险。综上所述，我们的数据可以作为监测稻田生态系统中肟菌酯残留的有用工具，也为深入了解该杀菌剂造成的环境行为和生态风险提供基础。 (C) 2015 Elsevier B.V. 保留所有权利。
Trifloxystrobin, a strobilurin fungicide, has beenwidely applied to control fungal diseases in various crops, especially in rice cultivation. However, its residual profile in rice paddy thatwas highly linked to its ecological risk still remains poorly understood. To elucidate the fate and primary metabolismof trifloxystrobin in rice paddy, a simple and efficient analyticalmethod was developed using the DisQuE extraction kit combined with GC-mu ECD and GC-EI-MS/MS analyses. As a result, methodological recoveries of trifloxystrobin fortified in paddywater, soil and rice strawranging from0.005 to 2 mg kg(-1) (mg L-1 forwater) were acquired from87.6% to 109.1% with relative standard deviation (RSD) from 1.9% to 9.5% (n= 5), and the limit of detection (LOD, signal to noise (S/N) = 3) and the limit of quantification (LOQ, S/N = 10) were 6.3 x 10(-4) mg L-1 and 2.09 x 10(-3) mg L-1, respectively, which indicates the favorable accuracy, precision and sensitivity of the method for effective monitoring of the trace amounts of residual trifloxystrobin in the rice paddy. Furthermore, dissipation of residual trifloxystrobin was in accordance with the first-order rate equation, showing the half-lives from 0.7 to 7.5 days, illustrating that trifloxystrobin generally degraded in a rapid rate in the rice paddy. Additionally, trifloxystrobin acid identified as the primary metabolite of trifloxystrobin in the rice paddy via GC-EI-MS/MS analysis was found to be dominantly accumulated in the paddy water and maintained up to 2.41 mg L-1 within 14 days, suggesting that long-termand frequent application of this fungicide may pose a high risk towards aquatic organisms in surrounding aqueous ecosystems through paddy drainage. Taken together, our data serve as a useful tool for monitoring residual trifloxystrobin in rice paddy ecosystem and also provide a basis for in-depth understanding of environmental behavior and ecological risk posed by this fungicide. (C) 2015 Elsevier B.V. All rights reserved.