Drivers and patterns of microbial community assembly in a Lyme disease vector
Drivers and patterns of microbial community assembly in a Lyme disease vector
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DOI：
10.1002/ece3.5361
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发表时间：
2019-07-01
影响因子：
2.6
通讯作者：
Swei, Andrea
中科院分区：
文献类型：
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作者：
Couper, Lisa I.;Kwan, Jessica Y.;Swei, Andrea
病媒传播的疾病构成了一个主要的全球健康负担，并且在地理范围和流行程度上都在增加。越来越多的证据表明，媒介微生物组可以影响病原体动力学，使微生物组成为媒介传播疾病生态学的焦点。然而，由于缺乏对媒介微生物组形成过程及其相互作用的基本理解，在研究和系统中推广初步发现并将这些发现转化为疾病控制策略的努力受到阻碍。在这里，我们使用16S rRNA测序并应用群落生态学框架来分析美国西部莱姆病媒介太平洋伊蚊（Ixodes pacificus）的微生物群落组装和相互作用。研究发现，垂直传播途径驱动太平洋赤潮微生物多样性和组成的种群水平模式，但微生物功能和总体丰度不随时间或卵群之间变化。此外，我们发现太平洋I.菌群的结构不是基于竞争的，而是基于非随机组合的，这可能是由于媒介特异性过滤过程，该过程在很大程度上消除了除立克次体外的所有内共生体。在太平洋壁虱个体的规模上，我们发现了一个高度有限的内部微生物群落的支持，并假设壁虱内共生体可能是影响病原体动态的媒介微生物组中最重要的组成部分。
Vector-borne diseases constitute a major global health burden and are increasing in geographic range and prevalence. Mounting evidence has demonstrated that the vector microbiome can impact pathogen dynamics, making the microbiome a focal point in vector-borne disease ecology. However, efforts to generalize preliminary findings across studies and systems and translate these findings into disease control strategies are hindered by a lack of fundamental understanding of the processes shaping the vector microbiome and the interactions therein. Here, we use 16S rRNA sequencing and apply a community ecology framework to analyze microbiome community assembly and interactions in Ixodes pacificus, the Lyme disease vector in the western United States. We find that vertical transmission routes drive population-level patterns in I. pacificus microbial diversity and composition, but that microbial function and overall abundance do not vary over time or between clutches. Further, we find that the I. pacificus microbiome is not strongly structured based on competition but assembles nonrandomly, potentially due to vector-specific filtering processes which largely eliminate all but the dominant endosymbiont, Rickettsia. At the scale of the individual I. pacificus, we find support for a highly limited internal microbial community, and hypothesize that the tick endosymbiont may be the most important component of the vector microbiome in influencing pathogen dynamics.