Molecular cloning of a novel transmembrane protein MOLT expressed by mature oligodendrocytes
Molecular cloning of a novel transmembrane protein MOLT expressed by mature oligodendrocytes
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DOI：
10.1093/jb/mvg135
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发表时间：
2003-08-01
影响因子：
2.7
通讯作者：
Ninomiya, Y
中科院分区：
文献类型：
--
作者：
Nomoto, H;Yonezawa, T;Ninomiya, Y
从脑毛细血管内皮细胞中分离出一种新型少突胶质细胞 (OL) 特异性 cDNA 并进行表征。该cDNA编码1099个氨基酸的蛋白质，含有信号肽和跨膜结构域。该蛋白在体内和体外细胞培养物中在成熟OL中表达，因此被命名为成熟OL跨膜蛋白(MOLT)。 RT-PCR分析显示MOLT mRNA在脑、肺、胰腺和睾丸中表达。针对小鼠 MOLT 的一部分产生的多克隆抗体与多极 OL 发生特异性反应，该多极 OL 具有渗透到灰质的径向定向过程。在白质中检测到更多的细胞，并且这些细胞具有纵向的过程。在大鼠 OL 谱系培养系统中，少突胶质细胞前体细胞最初不产生 MOLT mRNA 和蛋白质，但当它们开始分化为成熟 OL 时，它们开始表达 MOLT。因此，MOLT 可能在 OL 成熟时发挥作用，并可作为 OL 分化的细胞表面标记。
A novel oligodendrocyte (OL)-specific cDNA was isolated from brain capillary endothelial cells and characterized. The cDNA encodes a protein of 1099 amino acids that contains a signal peptide and a transmembrane domain. The protein was expressed in mature OLs in vivo and in vitro cell cultures and was thus designated as mature OL transmembrane protein (MOLT). RT-PCR analysis showed that MOLT mRNA was expressed in brain, lung, pancreas, and testis. A polyclonal antibody raised against a part of the mouse MOLT reacted specifically with multipolar OLs possessing radially oriented processes that penetrated into the gray matter. More cells were detected in the white matter, and these had longitudinally oriented processes. In a rat OL lineage culture system, oligodendrocyte precursor cells did not initially produce MOLT mRNA and protein, but when they begun to differentiate into mature OLs, they started expressing MOLT. Consequently, MOLT may function as OLs become mature and may serve as a cell-surface marker for OL differentiation.