Temporal dynamics of the cellular, humoral and cytokine responses in chickens during primary and secondary infection with Salmonella enterica serovar Typhimurium
Temporal dynamics of the cellular, humoral and cytokine responses in chickens during primary and secondary infection with Salmonella enterica serovar Typhimurium
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DOI：
10.1080/03079450310001636282
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发表时间：
2004-02-01
期刊：
影响因子：
2.8
通讯作者：
Smith, AL
中科院分区：
文献类型：
--
作者：
Beal, RK;Powers, C;Smith, AL
鼠伤寒沙门氏菌（S.鼠伤寒沙门氏菌）感染引起幼鸡全身性疾病，而在老鸡中，感染主要限于肠道。经口感染S.监测6周龄鼠伤寒（F98）和10周后再感染鼠伤寒的抗体产生、T细胞增殖和选定细胞因子（干扰素-γ、白细胞介素-1 β和转化生长因子-β（4））的产生。一个强有力的协调抗原特异性体液和细胞免疫应答与原发感染的消退有时间上的联系。在原发性感染的早期阶段，编码细胞因子、白细胞介素-1 β、转化生长因子-β（4）和干扰素-γ的mRNA水平也明显升高。继发感染局限于肠道，持续时间短于原发感染。脾免疫应答没有被继发感染进一步增强;实际上，抗原特异性增殖在继发感染后1天显著降低，这可以解释为反应性T细胞从脾运输到肠。
Salmonella enterica serovar Typhimurium ( S. Typhimurium) infections cause systemic disease in the young chick, whereas in the older chicken the infection is mainly restricted to the intestine. Chickens infected orally with S. Typhimurium ( F98) at 6 weeks of age and re- infected 10 weeks later were monitored for antibody production, T- cell proliferation and production of selected cytokines ( interferon-gamma, interleukin- 1beta and transforming growth factor- beta(4)). A strong coordinated antigen- specific humoral and cellular immune response was temporally linked to resolution of the primary infection. Enhanced levels of mRNA encoding the cytokines, interleukin- 1beta, transforming growth factor- beta(4) and interferon-gamma were also evident during early phases of primary infection. Secondary infection was restricted to the intestine and of shorter duration than primary infection. Splenic immune responses were not further enhanced by secondary infection; indeed, antigen- specific proliferation was significantly reduced at 1 day after secondary infection, which may be interpreted as the trafficking of reactive T cells from the spleen to the gut.