Cocirculation of multiple hantaviruses in Texas, with characterization of the small (S) genome of a previously undescribed virus of cotton rats (Sigmodon hispidus)
Cocirculation of multiple hantaviruses in Texas, with characterization of the small (S) genome of a previously undescribed virus of cotton rats (Sigmodon hispidus)
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DOI：
10.4269/ajtmh.1996.55.672
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发表时间：
1996-12-01
影响因子：
3.3
通讯作者：
Hjelle, B
中科院分区：
文献类型：
--
作者：
Rawlings, JA;TorrezMartinez, N;Hjelle, B
1995年5月，在德克萨斯州聋人史密斯县发生一例致命的儿童汉坦病毒肺综合征病例后，进行了环境和实验室调查。进行了诱捕行动，以确定可能的鼠患携带者。采集了6种鼠类和异种鼠类，每种鼠类中至少发现1份汉坦病毒阳性标本。对11例血清阳性标本进行了聚合酶链式反应(PCR)和病毒遗传物质测序。主要的汉坦病毒是El Moro Canyon病毒(ELMCV)，它发生在3只收获小鼠(Reithrodontomys Megalotis)和3只受检的鹿小鼠(Permyscus Manulatus)身上。在1只鹿小鼠和1只白足小鼠(P.leucopus)体内发现了辛诺布雷病毒(SNV)。1只血清阳性的家鼠(Mus Musculus)经聚合酶链式反应检测为阴性。两只棉鼠(Sigmodon Hispidus)被一种与Bayou汉坦病毒最相似的新型病毒(Mulushe病毒[MULEV])感染。测定了一株MULEV的全基因组小片段序列，并在大肠杆菌中高效表达了其核衣壳蛋白。血清学研究表明，人类感染最有可能的病原体是SNV。
An environmental and laboratory investigation was conducted after a fatal childhood case of hantavirus pulmonary syndrome occurred in Deaf Smith County, Texas in May 1995. A trapping campaign was conducted to identify possible rodent carriers. Six species of murid and heteromyid rodents were collected, and at least one hantavirus-seropositive specimen was found in each of the five murid species. Tissues from a selection of 11 seropositive specimens were examined by the polymerase chain reaction (PCR) and sequencing of viral genetic material. The predominant hantavirus was El Moro Canyon virus (ELMCV), which occurred in three of three harvest mice (Reithrodontomys megalotis) and in three of four deer mice (Peromyscus maniculatus) examined. Sin Nombre virus (SNV) was found in one deer mouse and one white-footed mouse (P. leucopus). A seropositive house mouse (Mus musculus) was negative by PCR. Two cotton rats (Sigmodon hispidus) were infected by a virus of novel genotype (Muleshoe virus [MULEV]) that bears closest resemblance to Bayou hantavirus. The sequence of the complete small genomic segment was determined for one MULEV, and high-level expression of its nucleocapsid protein was induced in Escherichia coli. Serologic studies indicated that the most likely etiologic agent in the human infection was SNV.