Localization and Quantitation of β-Endorphin in Human Brain and Pituitary
Localization and Quantitation of β-Endorphin in Human Brain and Pituitary
复制标题
人脑和垂体中 β-内啡肽的定位和定量
DOI：
--
复制
发表时间：
1980
期刊：
影响因子：
--
通讯作者：
S. Yen
中科院分区：
文献类型：
--
作者：
M. Wilkes;W. Watkins;R. Stewart;S. Yen
采用特异性放射免疫法（RIA）测定下丘脑各核区、下丘脑外脑区和垂体前叶和后叶中人β-内啡肽（βh-EP）的浓度。弓形核（169±35 pg/100 μg蛋白，n = 7）和正中隆起（163±32 pg/100 μg蛋白，n = 6）的βh-EP浓度在17个脑区中最高。凝胶排斥色谱法测定，下丘脑的免疫反应性βh-EP与真实βh-EP相对应。经层析和RIA检测，垂体前部（1.53 × 105±0.51 × 105 pg/100 μg蛋白，n = 3）和后部（1.41 × 105±0.38 × 105 pg/100 μg蛋白，n = 5）的βh-EP浓度比下丘脑高约1000倍。经免疫细胞化学判断，垂体内βh-EP分布于整个前叶、中间部和最靠近中间部的后叶部分。许多血管周围可见密集的免疫细胞化学染色。βh-EP和促肾上腺皮质激素（ACTH）在同一垂体细胞中共定位。目前的数据代表了βh-EP在人脑和人类垂体的单独叶中的第一个明确的定位和定量。
The concentration of human β-endorphin (βh-EP) was measured in various hypothalamic nuclei, in extra-hypothalamic brain regions and in the anterior and posterior lobes of the pituitary using a specific radioimmunoassay (RIA). The βh-EP concentrations in the arcuate nucleus (169 ± 35 pg/100 μg protein, n = 7) and median eminence (163 ± 32 pg/100 μg protein, n = 6) were among the highest in the 17 brain areas examined. The immunoreactive βh-EP in the hypothalamus corresponded to authentic βh-EP, as determined by gel exclusion chromatography. By chromatography and RIA the βh-EP concentrations in anterior (1.53 × 105 ±0.51 × 105 pg/100 μg protein, n = 3) and posterior (1.41 × 105 ± 0.38 × 105 pg/100 μg protein, n = 5) pituitary were found to be approximately 1,000-fold higher than in hypothalamus. Within the pituitary βh-EP was localized throughout the anterior lobe, in the pars intermedia and in that part of the posterior lobe nearest the pars intermedia, as judged by immunocytochemistry. Dense immunocytochemical staining was found along the perimeter of many blood vessels. βh-EP and adrenocorticotropin (ACTH) were co-localized in the same pituitary cells. The present data represent the first unequivocal localization and quantitation of βh-EP in human brain and in the separate lobes of the human pituitary.