Morphogenesis of flattened unifacial leaves inJuncus prismatocarpus(Juncaceae)
Morphogenesis of flattened unifacial leaves inJuncus prismatocarpus(Juncaceae)
复制标题
灯心草（灯心科）扁平单面叶的形态发生
DOI：
10.1111/nph.15649
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发表时间：
2019
期刊：
影响因子：
9.4
通讯作者：
Tsukaya Hirokazu
中科院分区：
文献类型：
--
作者：
Yin Xiaofeng;Tsukaya Hirokazu
为了揭示扁化单面叶的形态发生模式，我们分析了柱果灯心草（Juncus prismatocarpus）叶片细胞分裂的方向和分布，利用脉冲追踪5-乙炔基-2 ′-脱氧尿苷（5-ethynyl-2′-deoxyuridine）方法，对柱果灯心草叶片细胞分裂的方向进行了定量和定位，并将加厚细胞分裂的分布与下垂叶（DROOPING LEAF，DL）的表达模式进行了比较，一个与叶片加厚有关的关键基因，在柱果茉莉叶片的所有细胞分裂方向中，从质体时程2期早期到质体时程3期，加厚细胞分裂最丰富（> 45%）。细胞分裂图谱表明，在特定方向上的细胞分裂并不局限于特定的域，而是扩散地分布在叶片的整个横截面积上。梯度分析表明，近轴区加厚细胞分裂的分布较远轴区密集。相反的加厚细胞分裂的长期和弥漫性的分布，DL表达是短暂的，并限制在一个狭窄的bands.Our结果表明，一个弥漫的“加厚分生组织”在扁平单面叶的发展中起着关键作用。
To reveal the mode of morphogenesis of flattened unifacial leaves, we analysed the cell division direction and distribution on the leaf blade ofJuncus prismatocarpus.Using the pulse–chase 5‐ethynyl‐2′‐deoxyuridine method, we quantified and mapped the cell division direction on the leaf blade ofJ. prismatocarpusand compared the distribution of thickening cell divisions with the expression pattern ofDROOPING LEAF(DL), a key gene involved in leaf blade thickening.Thickening cell divisions were the most abundant (> 45%) among all cell division directions on the leaf blade ofJ. prismatocarpusfrom the early plastochron 2 stage through the plastochron 3 stage. Mapping of cell divisions indicated that cell divisions in a particular direction were not restricted to a particular domain but were distributed diffusely throughout the entire cross‐sectional area of the leaf blade. Gradient analysis indicated that the distribution of thickening cell divisions of the adaxial domain was denser than that of the abaxial domain. Contrary to the prolonged and diffuse distribution of thickening cell divisions,DLexpression was transient and restricted in a narrow band.Our results suggest that a diffuse ‘thickening meristem’ plays the key role in the development of flattened unifacial leaves.