The long-range restriction map surrounding the mouse agouti locus reveals a disparity between physical and genetic distances.
The long-range restriction map surrounding the mouse agouti locus reveals a disparity between physical and genetic distances.
复制标题
小鼠刺豚鼠基因座周围的远程限制图谱揭示了物理距离和遗传距离之间的差异。
DOI：
10.1016/0888-7543(89)90080-3
复制
发表时间：
1989
期刊：
影响因子：
4.4
通讯作者：
Epstein,CJ
中科院分区：
文献类型：
--
作者：
Barsh,GS;Epstein,CJ
Isolation of a gene based on its location, which depends on aligning physical landmarks with the genetic map, can yield basic information about genome structure and organization. As a first step toward isolating the mouse agouti (A) locus, we have begun to define the physical position of this gene relative to genetically linked DNA probes from thePsp, Emv-15, andSrcloci. Using a combination of pulsed-field gel techniques that include partial digestion with rare-cutting restriction enzymes and analysis of polymorphic sites present in certain inbred strains, we have constructed long-range restriction maps for each of the probes that span a total of more than 3000 kb. TheSrcandEmv-15probes are less than 600 kb apart, but are separated from thePspprobe by at least 1500 kb. By determining the position of a 75-kb deletion that inactivates agouti function, we have localized theAlocus to within 500 kb of thePspprobe, but more than 600 kb away from theEmv-15probe. These physical distances contrast with the known recombination frequencies, 3±3 cM forA-Pspand <0.3 cM forA-Emv-15, and suggest that recombination betweenAandEmv-15may be suppressed.
