Phosphate deprivation inhibits NH4+ transport in OK cells.
Phosphate deprivation inhibits NH4+ transport in OK cells.
复制标题
缺磷会抑制 OK 细胞中 NH4 的转运。
DOI：
10.1016/0005-2736(93)90214-k
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Kempson,SA
中科院分区：
文献类型：
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作者：
Chen,JG;Kempson,SA
膳食磷酸盐的去除导致大鼠尿铵排泄减少，这可能是通过近端小管中铵转运的改变介导的。在本研究中，OK肾上皮细胞系，近端小管的模型，用于确定是否NH 4+转运在急性磷酸盐剥夺改变。用NH 4Cl溶液灌流后的细胞内pH值计算细胞内NH 4+浓度。在NH 4Cl酸化的前2 min，胞内[NH 4+]呈线性增加. NH 4+转运（由胞内[NH 4+]初始增加速率定义）在磷酸盐剥夺的细胞中减少了32%（P< 0.005）。氯化钡可抑制NH 4+的转运，而DIDS、阿米洛利和哇巴因则不抑制NH 4+的转运，提示NH 4+的转运可能是通过K+通道进行的。急性缺磷可能通过降低细胞膜K+通透性而抑制OK细胞NH 4+转运.
Deprivation of dietary phosphate leads to a decrease in urinary excretion of ammonium in rats which may be mediated through an alteration in ammonium transport in the proximal tubule. In the present study the OK renal epithelial cell line, a model for the proximal tubule, was used to determine if NH 4+ transport was changed during acute phosphate deprivation. The intracellular pH after perfusion with NH 4 Cl solution was used for calculation of intracellular NH 4+ concentration. Intracellular [NH 4+] increased linearly during the first 2 min of acidification with NH 4 Cl. NH 4+ transport, defined by the initial rate of the increase in intracellular [NH 4+], was decreased by 32%(P< 0.005) in phosphate-deprived cells. This transport process was inhibited by barium chloride, but not by DIDS, amiloride or ouabain, suggesting that the NH 4+ transport pathway may utilize K+ channels. Acute phosphate deprivation may inhibit NH 4+ transport in OK cells by decreasing membrane K+ permeability.