Hypertriglyceridemic serum, very low density lipoprotein, and iron enhance Mycobacterium avium replication in human macrophages.
Hypertriglyceridemic serum, very low density lipoprotein, and iron enhance Mycobacterium avium replication in human macrophages.
复制标题
高甘油三酯血清、极低密度脂蛋白和铁可增强鸟分枝杆菌在人巨噬细胞中的复制。
DOI：
10.1093/infdis/170.5.1248
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Tsuchida,N
中科院分区：
文献类型：
--
作者：
Douvas,GS;May,MH;Pearson,JR;Lam,E;Miller,L;Tsuchida,N
Fe ~（3+）和血清脂质可促进鸟分枝杆菌7497（血清型4）在人巨噬细胞中的生长。向在正常血清中培养的巨噬细胞中加入铁（1-80µg/mL）导致生长增加10倍。如果补充铁的巨噬细胞在感染后高尿酸血症供体的血清中培养，M。鸟的生长增加了103- 104倍。在没有巨噬细胞的情况下，未观察到血清之间细菌生长的差异。去除极低密度脂蛋白（VLDL）消除血清之间的差异。从高脂血症血清中分离的VLDL导致生长比从正常血清中观察到的VLDL增加105倍。加速M。加入超氧化物歧化酶（1000 IU/mL）可部分抑制高脂血症血清培养的巨噬细胞的增殖。结果表明，铁刺激O2诱导的VLDL氧化及其随后在巨噬细胞中的积累。由此产生的铁和脂质负载细胞成为分枝杆菌生长的极好宿主。
The growth ofMycobacterium avium7497, serovar 4, in cultured human macrophages is enhanced by Fe3+and serum lipids over 7 days. Iron (1–80µg/mL)added to macrophages cultured in normal serum resulted in 10-fold increases in growth. If iron-supplemented macrophages were cultured in serum from hypertriglyceridemic donors after infection,M. aviumgrowth increased 103- to 104-fold. Without macrophages, differences in bacterial growth between sera were not seen. Removal of very low density lipoprotein (VLDL) eliminated the differences between sera. Isolated VLDL from hyperlipidemic serum resulted in 105-fold increases in growth over that seen with VLDL from normal sera. AcceleratedM. aviumgrowth in macrophages cultured with hyperlipidemic serum was partly inhibited by the addition of superoxide dismutase (1000 IU/mL). Results suggest that iron stimulates O2-induced oxidation of VLDL and its subsequent accumulation in macrophages. The resultant iron- and lipid-laden cells become excellent hosts for mycobacterial growth.