In silico analyses of mouse inner-ear transcripts
In silico analyses of mouse inner-ear transcripts
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DOI：
10.1007/s10162-002-2058-2
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发表时间：
2003-03-01
影响因子：
2.4
通讯作者：
Dahl, HHM
中科院分区：
文献类型：
--
作者：
Klockars, T;Perheentupa, T;Dahl, HHM
内耳的发育和功能是复杂的，需要许多基因的正确和协调表达。人类和其他基因组分析的最新进展为识别与听力有关的基因提供了工具。随着越来越多的核苷酸序列信息的积累，分子生物学的实验方法正在迅速地被计算方法所补充和部分取代。在这项研究中，我们提出了全面的计算机分析的cDNA文库代表近1600个转录分离小鼠内耳。通过挖掘公共数据库，我们能够快速有效地识别出许多可能在内耳的听觉或前庭功能中具有特定作用的转录本。分析揭示了大约600个已知基因和近100个内耳特异性转录本。根据染色体定位和内耳表达模式，其中近50个是听力障碍的候选基因。我们描述了一种强有力的方法来识别与听力和前庭功能相关的新基因，进一步增加了我们对内耳分子生物学的理解。
The development and function of the inner ear is complex requiring the correct and coordinated expression of many genes. The recent progress in the analyses of the human and other genomes has provided tools for identification of genes involved in hearing. As more and more nucleotide sequence information accumulates, experimental methods of molecular biology are rapidly being supplemented, and partially supplanted, by computational methods. In this study we present comprehensive in silico analyses of a cDNA library representing almost 1600 transcripts isolated from mouse inner ear. By mining the public databases we were able to rapidly and efficiently identify numerous transcripts likely to have a specific role in the auditory or vestibular function of the inner ear. Analyses revealed about 600 known genes and almost 100 inner-ear specific transcripts. Almost 50 of these are candidate genes for hearing impairment based on their chromosomal localization and inner-ear expression pattern. We describe a powerful approach to identify novel genes associated with hearing and vestibular function, further increasing our understanding of the molecular biology of the inner ear.