Cutin, cutinase and esterase as related to the induced systemic resistance of cucumber against Colletotrichum lagenarium.
Cutin, cutinase and esterase as related to the induced systemic resistance of cucumber against Colletotrichum lagenarium.
复制标题
角质、角质酶和酯酶与黄瓜对炭疽菌的诱导系统抗性有关。
DOI：
10.1006/pmpp.1995.1017
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发表时间：
1995
影响因子：
2.7
通讯作者：
J. Kuc
中科院分区：
文献类型：
--
作者：
Q. Huang;J. Kuc
摘要从未免疫（对照）和用黄瓜炭疽菌分生孢子浸润第1叶系统免疫的黄瓜叶片2和3中分离出黄瓜叶片角质。在液体培养基中以切片为唯一碳源，研究了切片对C. lagenarium进行了比较。C. Lagenarium在含有分离自对照植物叶的角质的培养基上比在含有来自免疫植物叶的角质的培养基上生长得更好。真菌生长的差异在接种培养基后9、12和15天具有统计学显著性。在统计学上，真菌酯酶和角质酶的活性在培养基中含有切割不明显的差异。C.还研究了lagenarium对对照和系统免疫的黄瓜叶片的影响。含分生孢子的水滴中酯酶活性上升较快。从对照植物的叶片中回收的lagenarium与从免疫植物的叶片中回收的lagenarium相比。我们的数据表明，角质从免疫叶片可能会水解更大的困难，由真菌和角质和/或其他叶表面成分免疫叶片可能会抑制活性或减少真菌酯酶的生产。
Abstract Cucumber leaf cutin was isolated from leaves 2 and 3 of unimmunized (control) plants and plants systemically immunized by infiltrating leaf 1 with conidia of Colletotrichum lagenarium . The cutins were used as sole carbon sources in a liquid medium, and their effects on the growth and production of cutinase and esterase by C. lagenarium were compared. C. lagenarium grew better on the medium containing cutin isolated from leaves of control plants than on the medium containing cutin from leaves of immunized plants. The differences in fungal growth were statistically significant 9, 12 and 15 days after inoculation of the media. Statistically, significant differences were not apparent in fungal esterase and cutinase activities in media containing the cutins. The activity of esterase produced by C. lagenarium on control and systemically-immunized cucumber leaves was also studied. Esterase activity increased sooner in water droplets containing conidia of C. lagenarium retrieved from leaves of control plants as compared with those from leaves of immunized plants. Our data suggest that cutin from immunized leaves may be hydrolysed with greater difficulty by the fungus and cutin and/or other leaf surface components in immunized leaves may inhibit activity or reduce production of fungal esterase.