Polyethylene glycol additives reduce hemolysis in red blood cell suspensions exposed to mechanical stress
Polyethylene glycol additives reduce hemolysis in red blood cell suspensions exposed to mechanical stress
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DOI：
10.1097/01.mat.0000084176.30221.cf
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发表时间：
2003-09-01
期刊：
影响因子：
4.2
通讯作者：
Borovetz, HS
中科院分区：
文献类型：
--
作者：
Kameneva, MV;Repko, BM;Borovetz, HS
在循环辅助装置和其它血液接触系统的开发和使用中，对血细胞的机械损伤是相当令人关注的。此外，在体外循环和透析期间使用盐水、葡聚糖和其他血浆膨胀剂进行血液稀释会增加红细胞（RBC）对与这些程序相关的高剪切应力的敏感性。在本文中，我们提出聚乙二醇（PEG）作为一种潜在的红细胞保护剂，对机械诱导的细胞损伤。牛红细胞经受通过将不锈钢丸滚动通过红细胞悬浮液诱导的机械应力，持续恒定的暴露时间。在30%血细胞比容下，在各种介质中制备混悬液：PEG（20，000分子量）、自体牛血浆、右旋糖酐40溶液和磷酸盐缓冲盐水（PBS）。在与PEG混悬液相似的粘度下制备右旋糖酐40中的RBC混悬液。我们发现悬浮在血浆和PEG溶液中的RBC的溶血水平比在葡聚糖和PBS溶液中低几倍（p < 0.001）。在自体血浆中的RBC悬浮液和2.0%PEG溶液中发生的溶血之间未发现统计学显著差异。与PBS或悬浮培养基中相同浓度的葡聚糖相比，即使低至0.1%的PEG浓度也使溶血减少了40%以上。我们的数据证明了PEG分子在减少红细胞机械损伤方面的功效，并表明在辅助循环、透析和其他RBC受到广泛机械应力的程序中使用PEG的潜力。
Mechanical damage to blood cells is of considerable concern in the development and use of circulatory assist devices and other blood contacting systems. Furthermore, hemodilution with saline, dextran, and other plasma expanders applied during extracorporeal circulation and dialysis increases red blood cell (RBC) susceptibility to the high shear stresses associated with these procedures. In this paper, we present polyethylene glycol (PEG) as a potential erythrocyte protective agent against mechanically induced cellular trauma. Bovine RBCs were subjected to mechanical stress induced by rolling stainless steel shots through RBC suspensions for a constant exposure time. The suspensions were prepared at a hematocrit of 30% in various media: PEG (20,000 molecular weight), autologous bovine plasma, Dextran 40 solution, and phosphate buffered saline (PBS). RBC suspensions in Dextran 40 were prepared at a viscosity similar to the PEG suspensions. We found the hemolysis level of RBCs suspended in plasma and in PEG solutions to be several times lower (p < 0.001) than in the Dextran and PBS solutions. No statistically significant difference was found between the hemolysis that occurred in suspensions of RBCs in autologous plasma and in 2.0% PEG solutions. Even PEG concentration as low as 0.1% reduced hemolysis by more than 40% compared with PBS or the same concentration of Dextran in suspension medium. Our data demonstrate the efficacy of PEG molecules in reducing mechanical trauma to erythrocytes and suggest the potential for using PEG in assisted circulation, dialysis, and other procedures where RBCs are subjected to extensive mechanical stress.