Histochemical studies of obstructive adenitis in human submandibular salivary glands. II. Lectin binding and keratin distribution in the lesions.
Histochemical studies of obstructive adenitis in human submandibular salivary glands. II. Lectin binding and keratin distribution in the lesions.
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人颌下唾液腺阻塞性腺炎的组织化学研究。
DOI：
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
M. Mori
中科院分区：
文献类型：
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作者：
M. Nakai;K. Tsukitani;Y. Tatemoto;N. Hikosaka;M. Mori
本文报道了人阻塞性颌下腺炎（SMG）的凝集素结合谱和角蛋白分布，并与正常SMG进行了比较。组织学上，SMG的阻塞性变化包括早期腺泡萎缩、导管样结构形成，以及晚期慢性阶段腺泡细胞消失和导管段扩张。使用以下凝集素：Con A（Glc，Man）、PNA（Gal，GalNAc）、RCA-1（Gal）、DBA（GalNAc）、SBA（Gal，Gal-NAc）、UEA-1（α-L-Fuc）和WGA（GlcNAC，NeuNAc）。萎缩的腺泡细胞中凝集素染色通常减少，除了SBA结合，并且不规则地分布在改变的腺泡和导管上皮中。DBA和UEA-1凝集素的结合在病变中沿着导管段的管腔边缘沿着特别强烈。免疫组化检测角蛋白的特点是在萎缩的腺泡细胞和所有的导管段，而正常的腺泡细胞，无论是浆液性或粘液性，染色强烈。
Lectin-binding profiles and keratin distribution in obstructive adenitis of human submandibular glands (SMGs) are reported and compared those of normal SMGs. Histologically, obstructive changes in the SMGs included acinar atrophy, duct-like structure formation in the early stage, and disappearance of acinar cells and dilation of ductal segments in the later, chronic stage. The following lectins were used: Con A (Glc, Man), PNA(Gal, GalNAc), RCA-I(Gal), DBA(GalNAc), SBA(Gal, Gal-NAc), UEA-I(alpha-L-Fuc) and WGA(GlcNAC, NeuNAc). Lectin staining in atrophic acinar cells was usually reduced except for SBA binding and was irregularly distributed in altered acinar and ductal epithelia. Binding of DBA and UEA-I lectins were particularly intense along the luminar borders of ductal segments in the lesions. Immunohistochemically detectable keratins were characterized by intense staining in atrophic acinar cells and in all the ductal segments, whereas normal acinar cells, either serous or mucous, were negative.