Inhibitory effects of soluble MD‐2 and soluble CD14 on bacterial growth
Inhibitory effects of soluble MD‐2 and soluble CD14 on bacterial growth
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可溶性 MD-2 和可溶性 CD14 对细菌生长的抑制作用
DOI：
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发表时间：
2010
影响因子：
2.6
通讯作者：
K. Tanamoto
中科院分区：
文献类型：
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作者：
T. Ohnishi;M. Muroi;K. Tanamoto
研究了可溶形式的内毒素受体分子sMD-2和sCD 14对细菌生长的影响。当大肠杆菌和枯草芽孢杆菌与sMD-2或sCD 14在37°C下孵育18小时时，通过活细胞计数和NADPH/NADH活性评价，这些细菌的生长受到显著抑制。缺乏LPS结合必需区域的sCD 14突变体（sCD 14 d57 - 64）不能抑制E.而该突变体抑制了B的生长。枯草芽孢杆菌向细菌培养物中加入过量PG可逆转sMD-2对B生长的抑制作用。而对E.杆菌此外，当通过ELISA进行评估时，sMD-2和sCD 14均特异性结合PG。综上所述，这些结果表明sMD-2和sCD 14抑制革兰氏阳性菌和革兰氏阴性菌的生长，并进一步表明与PG和LPS的结合分别参与了sMD-2对革兰氏阳性菌和sCD 14对革兰氏阴性菌的抑制作用。
The effects of the soluble forms of the endotoxin receptor molecules sMD‐2 and sCD14 on bacterial growth were studied. When Escherichia coli and Bacillus subtilis were incubated at 37°C for 18 hr with either sMD‐2 or sCD14, growth of these bacteria was significantly inhibited as evaluated by viable cell counts and NADPH/NADH activity. A mutant of sCD14 (sCD14d57‐64) lacking a region essential for LPS binding did not inhibit the growth of E. coli, whereas this mutant did inhibit the growth of B. subtilis. Addition of excess PG to the bacterial culture reversed the inhibitory effect of sMD‐2 on the growth of B. subtilis, but not on the growth of E. coli. Furthermore, when evaluated by ELISA, both sMD‐2 and sCD14 bound specifically to PG. Taken together, these results indicate that sMD‐2 and sCD14 inhibit the growth of both Gram‐positive and Gram‐negative bacteria and further suggest that binding to PG and LPS is involved in the inhibitory effect of sMD‐2 on Gram‐positive bacteria and of sCD14 on Gram‐negative bacteria, respectively.
影响因子：
20.3
作者：
Pugin, J;Stern-Voeffray, S;Dunn-Siegrist, I
通讯作者：
Dunn-Siegrist, I