GENOTYPIC AND PHENOTYPIC CHARACTERIZATION OF HIV-1 IN PATIENTS WITH PRIMARY INFECTION
GENOTYPIC AND PHENOTYPIC CHARACTERIZATION OF HIV-1 IN PATIENTS WITH PRIMARY INFECTION
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DOI：
10.1126/science.8356453
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发表时间：
1993-08-27
期刊：
影响因子：
56.9
通讯作者：
HO, DD
中科院分区：
文献类型：
--
作者：
ZHU, TF;MO, HM;HO, DD
更好地描述原发性感染患者的人类免疫缺陷病毒1型（HIV-1）对获得性免疫缺陷综合征（艾滋病）疫苗的开发具有重要意义，因为疫苗策略应该针对具有传播病毒特性的病毒分离株。在5个HIV-1血清转化者中，发现病毒表型均匀地嗜巨噬细胞和非合胞诱导。此外，这些病毒在每个患者体内的基因特征都是相同的，但在传播的病毒中没有一个共同的特征序列。在对性伴侣也进行研究的两个案例中，传播的病毒序列与传递者血液中的微小变异最吻合。与gp41、nef和p17相比，gp120中保存序列的压力更大，这表明一种选择性机制参与了传播。
Better characterization of human immunodeficiency virus-type 1 (HIV-1) in patients with primary infection has important implications for the development of an acquired immunodeficiency syndrome (AIDS) vaccine because vaccine strategies should target viral isolates with the properties of transmitted viruses. In five HIV-1 seroconverters, the viral phenotype was found to be uniformly macrophage-tropic and non-syncytium-inducing. Furthermore, the viruses were genotypically homogeneous within each patient, but a common signature sequence was not discernible among transmitted viruses. In the two cases where the sexual partners were also studied, the sequences of the transmitted viruses matched best with minor variants in the blood of the transmitters. There was also a stronger pressure to conserve sequences in gp120 than in gp41, nef, and p17, suggesting that a selective mechanism is involved in transmission.