Micro-Raman thermometry for measuring the temperature distribution inside the microchannel of a polymerase chain reaction chip
Micro-Raman thermometry for measuring the temperature distribution inside the microchannel of a polymerase chain reaction chip
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DOI：
10.1088/0960-1317/16/3/007
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发表时间：
2006-03-01
影响因子：
2.3
通讯作者：
Woo, SI
中科院分区：
文献类型：
--
作者：
Kim, SH;Noh, J;Woo, SI
首先使用显微拉曼光谱系统测量了微通道内的温度分布。微通道内的聚合酶链反应（PCR），这是非常敏感的温度变化，被选择来验证一种新的测温。采用硅微机械加工技术制作了微型PCR芯片。该微芯片由芯片内部的微通道、硅表面的铂膜加热器和铂膜传感器组成。利用显微拉曼光谱系统分析了水分子的O-H伸缩振动模式。其光谱变化随温度进行校准。氢键（HB）与非氢键（NHB）O-H伸缩振动模的强度比与温度呈线性关系。根据微通道的位置，控制在55 ℃的微通道的温度在53.6 ℃至54.8 ℃之间变化。
The temperature distribution inside the microchannel was first measured using a micro-Raman spectroscopy system. Polymerase chain reaction (PCR) inside the microchannel, which is very sensitive to temperature change, was chosen to validate a novel thermometry. The micro PCR chip was fabricated by silicon micromachining. The microchip consisted of a microchannel inside the chip, a Pt film heater and a Pt film sensor on the silicon surface. The O-H stretch vibration mode of water was analyzed using a micro-Raman spectroscopy system. Its spectral changes were calibrated with temperature. The intensity ratio of the hydrogen-bonded (HB) to non-hydrogen-bonded (NHB) O-H stretch vibration modes has a linear relation with temperature. The temperature of the microchannel controlled at 55 degrees C varied from 53.6 to 54.8 degrees C depending on the location of the microchannel.