Genetic structure and population variability of tomato yellow leaf curl China virus
Genetic structure and population variability of tomato yellow leaf curl China virus
复制标题
番茄黄曲叶病毒中国病毒的遗传结构和群体变异
DOI：
10.1128/jvi.02431-06
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发表时间：
2007-06-01
影响因子：
5.4
通讯作者：
Li, Hongye
中科院分区：
文献类型：
--
作者：
Ge, Linmei;Zhang, Jiangtao;Li, Hongye
双生病毒具有环状单链DNA基因组，是热带和亚热带地区的重要病原体，但其种群多样性和变异性知之甚少。在这里，我们已经调查了中国番茄黄化曲叶病毒（TYLCCNV），双生病毒科双生病毒属双生病毒的变异积累。在自然感染的番茄（Solanum lycopersicom）植物和用TYLCCNV DNA克隆群实验感染的本氏烟草（Nicotiana benthamiana）和番茄植物中分析群体变异，以提供用于感染起始的相同序列。我们的研究结果表明，TYLCCNV在自然感染的番茄植株中的群体是遗传异质性的，并且在从N. benthamiana和番茄植物已经感染了克隆DNA。TYLCCNV在这些植物中的群体的这一特征由共有序列和不相同但与共有序列密切相关的突变体库组成，并且与许多RNA病毒描述的准种概念一致。突变频率为10（-4）左右。benthamiana和番茄在接种后60天，一个值相当的植物RNA病毒的报道。TYLCCNV种群的准种性质表明，TYLCCNV能够快速进化和适应不断变化的农业实践。
Geminiviruses have circular single-stranded DNA genomes and are important pathogens in tropical and subtropical regions, but their population diversity and variability are poorly understood. Here, we have investigated variations accumulating in Tomato yellow leaf curl China virus (TYLCCNV), a geminivirus in the genus Begomovirus of the family Geminiviridae. The population variation was analyzed in a naturally infected tomato (Solanum lycopersicom) plant and in Nicotiana benthamiana and tomato plants experimentally infected with a swarm of TYLCCNV DNA clones to provide an identical sequence for initiation of infection. Our results demonstrate that the population of TYLCCNV in a naturally infected tomato plant was genetically heterogeneous and that rapid mutation occurred in the populations amplified from N. benthamiana and tomato plants that had been infected with cloned DNA. This feature of the population of TYLCCNV in these plants consisted of the consensus sequence and a pool of mutants that are not identical but are closely related to the consensus sequence, and it coincides with the quasispecies concept described for many RNA viruses. The mutation frequency was circa 10(-4) in N. benthamiana and tomato at 60 days postinoculation, a value comparable to that reported for plant RNA viruses. The quasispecies-like nature of the TYLCCNV populations suggested that TYLCCNV is capable of rapid evolution and adaptation in response to changing agricultural practices.