Thyroid hormone-induced sonic hedgehog signal up-regulates its own pathway in a paracrine manner in the Xenopus laevis intestine during metamorphosis.
Thyroid hormone-induced sonic hedgehog signal up-regulates its own pathway in a paracrine manner in the Xenopus laevis intestine during metamorphosis.
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DOI：
10.1002/dvdy.23723
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发表时间：
2012-02
影响因子：
2.5
通讯作者：
Ishizuya-Oka, Atsuko
中科院分区：
文献类型：
--
作者：
Hasebe, Takashi;Kajita, Mitsuko;Fu, Liezhen;Shi, Yun-Bo;Ishizuya-Oka, Atsuko
关键词：
在非洲爪蟾变态过程中，Sonic hedgehog（Shh）是由甲状腺激素（TH）在转录水平上直接诱导的，是肠道重构的最早期事件之一。然而，该信号通路的其他组分的调节仍有待分析。在这里，我们分析了时空表达Patched（Ptc）-1，Smoothened（Smo），Gli 1，Gli 2和Gli 3在自然和TH诱导的肠重塑。我们发现，所有的基因检查是瞬时上调间充质组织在肠变态。有趣的是，在蛋白质合成抑制剂的存在下，Gli 2而不是其他人被TH诱导，这表明Gli 2是一个直接的TH反应基因，而其他人可能是间接的。此外，我们通过器官培养实验证明，即使在TH不存在的情况下，Shh的过表达也增强了Ptc-1、Smo和Glis的表达，表明Shh在肠重塑过程中调节其自身的通路组分。
During Xenopus laevis metamorphosis, Sonic hedgehog (Shh) is directly induced by thyroid hormone (TH) at the transcription level as one of the earliest events in intestinal remodeling. However, the regulation of other components of this signaling pathway remains to be analyzed. Here, we analyzed the spatiotemporal expression of Patched (Ptc)-1, Smoothened (Smo), Gli1, Gli2 and Gli3 during natural and TH-induced intestinal remodeling. We show that all of the genes examined are transiently up-regulated in the mesenchymal tissues during intestinal metamorphosis. Interestingly, in the presence of protein synthesis inhibitors, Gli2 but not the others was induced by TH, suggesting that Gli2 is a direct TH response gene, while the others are likely indirect ones. Furthermore, we demonstrate by the organ culture experiment that overexpression of Shh enhances the expression of Ptc-1, Smo and Glis even in the absence of TH, indicating that Shh regulates its own pathway components during intestinal remodeling.
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