Phylogeographical, ecological and biological patterns shown by nuclear (ssrRNA and gGAPDH) and mitochondrial (Cyt b) genes of trypanosomes of the subgenus Schizotrypanum parasitic in Brazilian bats
Phylogeographical, ecological and biological patterns shown by nuclear (ssrRNA and gGAPDH) and mitochondrial (Cyt b) genes of trypanosomes of the subgenus Schizotrypanum parasitic in Brazilian bats
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DOI：
10.1016/j.ijpara.2009.08.015
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发表时间：
2010-03-01
影响因子：
4
通讯作者：
Teixeira, Marta M. G.
中科院分区：
文献类型：
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作者：
Cavazzana, Manzelio, Jr.;Marcili, Arlei;Teixeira, Marta M. G.
感染巴西蝙蝠的锥虫属物种的遗传多样性和地理分布模式进行了评估，检查1043蝙蝠从亚马逊河流域，潘塔纳尔，塞拉多和大西洋森林生物群系的巴西捕获的7个家庭的63种。感染锥虫的蝙蝠的患病率，估计由血培养，是12.9%，导致77个培养物的分离，大多数形态鉴定为锥虫cf。cruzi，根据ssrRNA基因部分序列进行条形码编码，将其归入裂锥菌亚属（Schizotrypanum），并鉴定为裂锥菌（T. cruzi（15）、T. cruzi marinkellei（37）或T.参见dionisii（25）.利用核ssrRNA、糖体甘油醛3-磷酸脱氢酶（gGAPDH）和线粒体细胞色素B（Cyt B）基因序列进行系统发育分析，得到3个分支，它们聚在一起形成裂锥虫亚属。除了媒介关联，蝙蝠锥虫与蝙蝠的进化历史、生态学和地理学有关。锥虫属dionisii锥虫（32.4%）感染了从巴西北部到南部的所有生物群落中捕获的来自4个蝙蝠科的12种蝙蝠，并与T. dionisii来自欧洲，尽管相隔一定的遗传距离。克氏锥虫marinkellei（49.3%）仅限于从亚马逊河流域到潘塔纳尔（北部到中部）的叶口类蝙蝠。克氏锥虫（18.2%）主要发现于来自潘塔纳尔/塞拉多和大西洋森林（中部至东南部）的蝙蝠和叶口类蝙蝠中，少数分离株来自亚马逊。(C)2009年澳大利亚寄生虫学会由爱思唯尔有限公司出版。保留所有权利。
The genetic diversity and phylogeographical patterns of Trypanosoma species that infect Brazilian bats were evaluated by examining 1043 bats from 63 species of seven families captured in Amazonia, the Pantanal, Cerrado and the Atlantic Forest biomes of Brazil. The prevalence of trypanosonne-infected bats, as estimated by haemoculture, was 12.9%, resulting in 77 Cultures of isolates, most morphologically identified as Trypanosoma cf. cruzi, classified by barcoding using partial sequences from ssrRNA gene into the subgenus Schizotrypanum and identified as T. cruzi (15), T cruzi marinkellei (37) or T. cf. dionisii (25). Phylogenetic analyses using nuclear ssrRNA, glycosomal glyceraldehyde 3-phosphate dehydrogenase (gGAPDH) and mitochondrial cytochrome b (Cyt b) gene sequences generated three clades, which clustered together forming the subgenus Schizotrypanum. In addition to vector association, bat trypanosomes were related by the evolutionary history, ecology and phylogeography of the bats. Tryponosoma cf. dionisii trypanosomes (32.4%) infected 12 species from four bat families captured in all biomes, from North to South Brazil, and clustered with T. dionisii from Europe despite being separated by some genetic distance. Trypanosoma cruzi marinkellei (49.3%) was restricted to phyllostomid bats from Amazonia to the Pantanal (North to Central). Trypanosoma cruzi (18.2%) was found mainly in vespertilionid and phyllostomid bats from the Pantanal/Cerrado and the Atlantic Forest (Central to Southeast), with a few isolates from Amazonia. (C) 2009 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.