Influence of water temperature, salinity, and pH on survival and growth of toxigenic Vibrio cholerae serovar 01 associated with live copepods in laboratory microcosms
Influence of water temperature, salinity, and pH on survival and growth of toxigenic Vibrio cholerae serovar 01 associated with live copepods in laboratory microcosms
复制标题
实验室微观世界中水温、盐度和 pH 对与活桡足类相关的产毒霍乱弧菌血清型 01 存活和生长的影响
DOI：
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发表时间：
1984
影响因子：
4.4
通讯作者：
R. Colwell
中科院分区：
文献类型：
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作者：
A. Huq;P. West;E. B. Small;M. Huq;R. Colwell
水温、盐度和pH值对霍乱弧菌O1血清型细胞增殖及其与活的浮游甲壳类动物的附着的影响，桡足类，通过使用实验室微宇宙进行了调查。通过将水温提高到30摄氏度，证明了对霍乱弧菌增殖的显著影响，以及细胞对活桡足类的附着。这些分别通过琼脂平板上的可培养物计数和扫描电子显微镜直接观察来测量。三个盐度检查（5，10和15%），最大的增长V. cholesterol和附着桡足类发生在15%。碱性pH值（8.5）是霍乱弧菌附着和增殖的最佳pH值，而pH值为6.5和7.5时，霍乱弧菌附着和增殖的最佳pH值均为8.5。它的结论是，条件影响附件霍乱弧菌血清型O1活桡足类在实验室条件下观察到的也可能发生在自然河口环境中，因此，霍乱的流行病学是显着的。
The influence of water temperature, salinity, and pH on the multiplication of toxigenic Vibrio cholerae serovar O1 cells and their attachment to live planktonic crustaceans, i.e., copepods, was investigated by using laboratory microcosms. By increasing water temperatures up to 30 degrees C, a pronounced effect on the multiplication of V. cholerae was demonstrated, as was attachment of the cells to live copepods. These were measured by culturable counts on agar plates and direct observation by scanning electron microscopy, respectively. Of the three salinities examined (5, 10, and 15%), maximum growth of V. cholerae and attachment to copepods occurred at 15%. An alkaline pH (8.5) was optimal both for attachment and multiplication of V. cholerae, as compared with pH 6.5 and 7.5. It is concluded that conditions affecting attachment of V. cholerae serovar O1 to live copepods observed under laboratory conditions may also occur in the natural estuarine environment and, thereby, are significant in the epidemiology of cholera.