Bioinformatics analysis of the expression and role of microRNA-221-3p in head and neck squamous cell carcinoma.
Bioinformatics analysis of the expression and role of microRNA-221-3p in head and neck squamous cell carcinoma.
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DOI：
10.1186/s12885-021-08039-5
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发表时间：
2021-04-12
期刊：
影响因子：
3.8
通讯作者：
Kang M
中科院分区：
文献类型：
--
作者：
Zhou Z;Wu W;Li J;Liu C;Xiao Z;Lai Q;Qin R;Shen M;Shi S;Kang M
头颈部鳞状细胞癌(HNSCC)是全球第六大常见癌症，与高发病率和高死亡率有关。然而，miR-221-3p的靶基因和参与HNSCC的潜在机制仍不清楚。因此，在本研究中，我们研究了miR-221-3p在HNSCC中的作用。采用RT-qPCR方法检测48例正常对照和21例HNSCC患者组织中miR-221-3p的差异表达。MicroRNA-221-3p(miR-221-3p)的过度表达与HNSCC的发生发展密切相关。我们还对癌症基因组图谱(TCGA)和基因表达总表(GEO)数据库中的癌症文献进行了荟萃分析，以估计miR-221-3p在HNSCC中的表达。用miRWalk和TCGA数据库预测HNSCC中miR-221-3p的靶基因，并通过基因本体论对其进行功能注释。最后，使用Spearman的分析来确定相关靶基因在HNSCC发展过程中的重要途径中的作用。我们观察到miR-221-3p在HNSCC中的表达显著高于正常组织，其总体受试者工作特征(SROC)为0.86(95%CI：0.83，0.89)。KEGG和GO综合分析预测miR-221-3p可能通过以下代谢途径参与HNSCC的发生发展。药物代谢-细胞色素P450、UGT1A7和MAOB可能是miR-221-3p发挥作用的重要基因。基于生物信息学分析，我们的结果表明miR-221-3p可以作为一种非侵入性和超敏的生物标志物用于诊断。因此，miR-221-3p可能是HNSCC发生发展过程中的一个极其重要的基因座。希望，更多的工作将验证它作为未来临床研究的目标的有效性。
Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer worldwide, associated with a high rate of morbidity and mortality. However, the target genes of miR-221-3p and the underlying mechanism involved in HNSCC are still not clear. Therefore, in the current study, we studied the role of miR-221-3p in the HNSCC. Tissues collected from 48 control and 21 HNSCC patients were processed to check the differential expression of miR-221-3p by RT-qPCR. Overexpression of microRNA-221-3p (miR-221-3p) is significantly correlated to the onset and progression of HNSCC. We also conducted the meta-analysis of the cancer literature from the cancer genome atlas (TCGA) and the Gene Expression Omnibus (GEO) database to estimate the expression of miR-221-3p in HNSCC. The miR-221-3p target genes in the HNSCC were predicted with the miRWalk and TCGA databases, and functionally annotated via the Gene Ontology. Finally, Spearman’s analysis was used to determine the role of the related target genes in important pathways involved in the development of HNSCC. We observed a significantly higher expression of miR-221-3p in HNSCC compared to the normal with a summary receiver operating characteristic (sROC) of 0.86(95% Cl: 0.83,0.89). The KEGG and GO comprehensive analysis predicted that miR-221-3p might be involved in the development of HNSCC through the following metabolic pathways, viz. Drug metabolism - cytochrome P450 UGT1A7 and MAOB may be important genes for the role of miR-221-3p. Based on bioinformatics analysis, our results indicate that miR-221-3p may be used as a non-invasive and hypersensitive biomarker in the diagnosis. Thus, it can be concluded that miR-221-3p may be an extremely important gene locus involved in the process of the deterioration and eventual tumorigenesis of HNSCC. Hopefully, additional work will validate its usefulness as a target for future clinical research.
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影响因子：
14.9
作者：
Kanehisa M;Goto S;Sato Y;Furumichi M;Tanabe M
通讯作者：
Tanabe M
头部和颈部鳞状细胞癌患者的血清诱导了治疗细胞中的microRNA和靶基因表达谱。
影响因子：
4.7
作者：
Allen B;Schneider A;Victoria B;Nunez Lopez YO;Muller M;Szewczyk M;Pazdrowski J;Majchrzak E;Barczak W;Golusinski W;Golusinski P;Masternak MM
通讯作者：
Masternak MM
DOI：
10.1158/1078-0432.ccr-08-3131
发表时间：
2009-04-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Avissar M;Christensen BC;Kelsey KT;Marsit CJ
通讯作者：
Marsit CJ
影响因子：
4.3
作者：
Gillies, Jana K.;Lorimer, Ian A. J.
通讯作者：
Lorimer, Ian A. J.
影响因子：
5
作者：
Cheng, Zhou;Xing, Dayuan
通讯作者：
Xing, Dayuan