Expression and Regulation of Lipocalin-Type Prostaglandin D Synthase in Rat Testis and Epididymis1
Expression and Regulation of Lipocalin-Type Prostaglandin D Synthase in Rat Testis and Epididymis1
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DOI：
10.1095/biolreprod.103.022079
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发表时间：
2004-04
期刊：
影响因子：
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通讯作者：
Huiyang Zhu;Hong Ma;H. Ni;Xinghong Ma;N. Mills;Zeng-Ming Yang
中科院分区：
文献类型：
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作者：
Huiyang Zhu;Hong Ma;H. Ni;Xinghong Ma;N. Mills;Zeng-Ming Yang
摘要Lipocalin-type prostaglandin D synthase（L-PGDS）是一种双功能蛋白，在许多物种的雄性生殖器官中表达。然而，L-PGDS在大鼠体内的表达及调控机制尚不清楚。本研究采用原位杂交和免疫组织化学方法研究了性成熟、去势和二甲烷磺酸乙酯（EDS）处理条件下大鼠睾丸和附睾中L-PGDS表达的区域化和调控。在性成熟的大鼠，L-PGDS的mRNA弱表达，仅在睾丸管周细胞，而L-PGDS免疫染色高度检测到的Leydig细胞产后70天。在性成熟过程中，L-PGDS mRNA在生后70天的附睾头、体和尾中高度表达。与正常成人附睾L-PGDS表达相比，去势后附睾头、体、尾L-PGDS mRNA表达和蛋白免疫组化染色均显著降低。丙酸替卡松处理诱导去势大鼠附睾中L-PGDS表达显著增加。与成年大鼠相比，EDS处理后L-PGDS mRNA和蛋白表达下调。丙酸睾酮处理可诱导EDS处理大鼠附睾中L-PGDS mRNA和蛋白表达增加。总之，去势和EDS处理均导致大鼠附睾中L-PGDS表达显著降低，而丙酸睾酮处理可诱导去势和EDS处理大鼠附睾中L-PGDS表达增加，表明睾酮可上调大鼠附睾中L-PGDS的表达。
Abstract Lipocalin-type prostaglandin D synthase (L-PGDS), a bifunctional protein, is expressed in the male reproductive organs of many species. However, the expression and regulation of L-PGDS in rat are still uncertain. The present study investigated the regionalization and regulation of L-PGDS expression in rat testis and epididymis by in situ hybridization and immunohistochemistry under the conditions of sexual maturation, castration, and ethylene dimethane sulfonate (EDS) treatments. In sexually mature rats, L-PGDS mRNA was weakly expressed only in the testicular peritubular cells, whereas L-PGDS immunostaining was highly detected in the Leydig cells by Day 70 postpartum. During sexual maturation, L-PGDS mRNA expression was highly detected in the caput, corpus, and cauda of the epididymis 70 days after birth. Compared with normal L-PGDS expression in adult epididymis, both L-PGDS mRNA expression and protein immunostaining were significantly reduced in the caput, corpus, and cauda epididymis after castration. Testosterone propionate treatment induced a significant increase of L-PGDS expression in the epididymis of castrated rats. Compared with adult rat epididymis, L-PGDS mRNA and protein expression was down-regulated after EDS treatment. Testosterone propionate treatment could induce an increase of L-PGDS mRNA and protein expression in the epididymis of EDS-treated rats. In conclusion, both castration and EDS treatments caused a significant decrease of L-PGDS expression in the epididymis, whereas testosterone propionate treatment could induce an increase of L-PGDS expression in the epididymis of both castrated and EDS-treated rats, indicating that L-PGDS expression in the rat epididymis can be up-regulated by testosterone.