ANTIHEMOPHILIC FACTOR ANTIGEN - LOCALIZATION IN ENDOTHELIAL CELLS BY IMMUNOFLUORESCENT MICROSCOPY
ANTIHEMOPHILIC FACTOR ANTIGEN - LOCALIZATION IN ENDOTHELIAL CELLS BY IMMUNOFLUORESCENT MICROSCOPY
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DOI：
10.1172/jci107469
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发表时间：
1973-01-01
影响因子：
15.9
通讯作者：
HOYER, JR
中科院分区：
文献类型：
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作者：
HOYER, LW;SANTOS, RPD;HOYER, JR
用兔抗人抗人血友病因子(AHF)的单特异性抗体进行免疫荧光研究，确定了抗血友病因子(AHF，因子)的组织定位。已在广泛的人体组织内皮细胞中发现了AHF抗原的特异染色。在动脉、毛细血管和静脉内皮细胞以及肝、脾血窦内皮细胞中观察到染色模式。特异性荧光仅限于肾、肝、脾、淋巴结、心肌和平滑肌、甲状腺、脐带和皮肤等部位的内皮细胞。吸收研究证实，这种染色是针对细胞中含有AHF抗原的蛋白质的。内皮细胞胞浆内的荧光显示表明，这些细胞合成了含有AHF抗原的蛋白质。
The tissue localization of antihemophilic factor (AHF, Factor VIII) has been determined by immunofluorescent studies using monospecific rabbit antibody to human AHF. Specific staining demonstrating AHF antigens has been identified in endothelial cells of a wide range of human tissues. The staining pattern was observed in endothelial cells of arteries, capillaries, and veins as well as the cells lining hepatic and splenic sinusoids. Specific fluorescence was limited to these endothelial cells in sections of kidney, liver, spleen, lymph node, cardiac and smooth muscle, thyroid, umbilical cord, and skin. Absorption studies established that the staining was specific for cells in which there were proteins that had AHF antigens. The demonstration of fluorescence within the cytoplasm of endothelial cells suggests that these cells synthesize proteins that have AHF antigens.Images