The conserved Ala37 in the ERF/AP2 domain is essential for, binding with the DRE element and the GCC box
The conserved Ala37 in the ERF/AP2 domain is essential for, binding with the DRE element and the GCC box
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DOI：
10.1016/j.febslet.2006.01.048
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发表时间：
2006-02-01
期刊：
影响因子：
3.5
通讯作者：
Zhou, HM
中科院分区：
文献类型：
--
作者：
Liu, Y;Zhao, TJ;Zhou, HM
从甘蓝型油菜（Brassica napus）中克隆了4个编码乙烯应答元件结合因子（ERF）/AP 2结构域的基因，这些基因均能受到低温、乙烯、干旱、高盐、脱落酸和茉莉酸等胁迫的诱导。这4个基因分别命名为BnDREBIII-1和BnDREBIII-4，它们的ERF/AP 2结构域高度同源，只有第37位氨基酸不同。BnDREBIII-1被证明能够结合脱水反应元件和GCC盒，并反式激活下游基因的表达，而BnDREBIII-4不能结合两者。进一步的研究结果表明，Ala 37可能在ERF/AP 2结构域的DNA结合或稳定性中起关键作用。(c)2006年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
Four AP2/EREBP genes encoding putative ethylene-responsive element binding factor (ERF)/AP2 domains were cloned from Brassica napus, and these genes could be induced by low temperature, ethylene, drought, high salinity, abscisic acid and jasmonate treatments. These four genes, named BnDREBIII-1 to BnDREBIII-4, were highly homologous and the 37th amino acid was the only difference among their ERF/AP2 domains. BnDREBIII-1 was demonstrated to be able to bind to both dehydration-responsive element and the GCC box and transactivate the expression of downstream genes, while BnDREBIII-4 could bind neither. Further results suggested that Ala37 might play a crucial role in the DNA binding or the stability of the ERF/AP2 domain. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.