Mapping and genetic characterization of loci controlling the restoration of male fertility in Ogura CMS radish
Mapping and genetic characterization of loci controlling the restoration of male fertility in Ogura CMS radish
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DOI：
10.1023/b:molb.0000018759.27223.02
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发表时间：
2004-02-01
影响因子：
3.1
通讯作者：
Lydiate, DJ
中科院分区：
文献类型：
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作者：
Bett, KE;Lydiate, DJ
利用3个萝卜群体(Bc1、F-2和R8)对Ogura不育系的恢复系进行了定位。3个恢复基因座Rf1、Rf2和Rf3均为显性恢复等位基因，且各为上位性。使用来自每个群体的数据将RF1映射到Rs1的上部区域。利用F-2和R8两个群体将Rf2定位到Rs2的中间。利用R8群体将Rf3定位到RS7的上部区域。Bc1和F2群体的标记分析和连锁图谱已在前面描述过(Bett和Lydiate，2003)。R8群体中114个标记基因座的得分使萝卜属基因组的新图谱与共识图谱整合在一起。将萝卜中Ogura CMS恢复的复杂遗传控制与以前在甘蓝型油菜中描述的更简单的遗传控制进行了比较。与三个恢复基因座中的每一个相关联的标记将允许对定义的恢复基因座的各种组合的定义的恢复系和保持系进行常规生成和验证。虽然小古拉不育系在萝卜上的恢复可能涉及到额外的基因座，但3个基因座和每个基因座的诊断标记的识别为通过在更多的群体中定位来建立这一性状的遗传控制的整体模型提供了坚实的基础。
Three Raphanus populations (BC1, F-2 and R8) each segregating for the restoration of Ogura CMS were used to map restorer loci. The three restorer loci, Rf1, Rf2 and Rf3, each exhibited dominant restoring alleles and were each mutually epistatic. Rf1 was mapped to the upper region of Rs1 using data from each population. Rf2 was mapped to the middle of Rs2 using both the F-2 and R8 populations. Rf3 was mapped to the upper region of Rs7 using the R8 population. The marker analysis and linkage mapping of the BC1 and F2 populations were described previously (Bett and Lydiate, 2003). Scoring at 114 marker loci in R8 population allowed a new map of the Raphanus genome to be integrated with the consensus map. The complex genetic control of the restoration of Ogura CMS in Raphanus is compared with the more simple genetic control of this trait previously described in B. napus. Markers linked to each of the three restorer loci will allow the routine generation and verification of defined restorer and maintainer lines for various combinations of defined restorer loci. Although the restoration of Ogura CMS in Raphanus probably involves additional loci, the identification of three loci and diagnostic markers for each provides a solid foundation for the development of a holistic model for the genetic control of this trait through mapping in additional populations.