Effects of a Saccharomyces cerevisiae culture on ruminal bacteria that utilize lactate and digest cellulose
Effects of a Saccharomyces cerevisiae culture on ruminal bacteria that utilize lactate and digest cellulose
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DOI：
10.3168/jds.s0022-0302(97)76148-4
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发表时间：
1997-09-01
影响因子：
3.5
通讯作者：
Martin, SA
中科院分区：
文献类型：
--
作者：
Callaway, ES;Martin, SA
本研究的目的是确定酵母（酿酒酵母）培养物对瘤胃细菌乳酸利用和纤维素消化的影响。反刍月形单胞菌HD4在含有5g/L DL-乳酸、胰蛋白酶解酪蛋白酶和酵母提取物的培养基中的生长分别被1和5%（体积/体积）酵母培养物滤液刺激7和15%。在不含胰蛋白酶或酵母提取物的培养基中，1%和5%酵母培养物滤液在5g/L DL-乳酸盐上刺激反刍月形单胞菌H18和埃氏巨球菌B159和T81的生长。通过向不含胰蛋白酶或酵母提取物的培养基中加入酵母培养物滤液来刺激琥珀酸纤维杆菌S85和白色瘤胃球菌B199在6g/L纤维二糖上的生长。酵母培养物滤液增加了由Sel.ruminantium HD 4产生的乙酸盐和总挥发性脂肪酸的浓度，并且增加了由Set产生的丙酸盐和总挥发性脂肪酸的浓度。ruminantium H18的终产物形成，但不改变M. Elsdenii或纤维素分解细菌。用酵母培养物处理能提高F.产琥珀酸菌S85和产黄瘤胃球菌FD 1。总的来说，这些结果表明酵母培养物提供可溶性生长因子（即，有机酸、B族维生素和氨基酸），其刺激利用乳酸盐和消化纤维素的瘤胃细菌的生长。
The objective of this study was to determine the effects of a yeast (Saccharomyces cerevisiae) culture on lactate utilization and cellulose digestion by ruminal bacteria. Growth of Selenomonas ruminantium HD4 in medium that contained 5 g/L of DL-lactate, Trypticase, and yeast extract was stimulated 7 and 15% by 1 and 5% (vol/vol) yeast culture filtrate, respectively. The 1 and 5% yeast culture filtrate stimulated growth of Selenomonas ruminantium H18 and Megasphaera elsdenii B159 and T81 on 5 g/L of DL-lactate in medium without Trypticase or yeast extract. Growth of Fibrobacter succinogenes S85 and Ruminococcus albus B199 on 6 g/L of cellobiose was stimulated by the addition of yeast culture filtrate to medium without Trypticase or yeast extract. The yeast culture filtrate increased the concentrations of acetate and total volatile fatty acids that were produced by Sel. ruminantium HD4 and increased the concentrations of propionate and tot al volatile fatty acids that were produced by Set. ruminantium H18 but did not alter end-product formation of M. elsdenii or cellulolytic bacteria. Treatment with yeast culture increased the initial rate but not the extent of cellulose digestion by F. succinogenes S85 and Ruminococcus flavefaciens FD1. Collectively, these results suggest that yeast culture provides soluble growth factors (i.e., organic acids, B vitamins, and amino acids) that stimulate growth of ruminal bacteria that utilize lactate and digest cellulose.