Intraclonal mating in Trypanosoma brucei is associated with out-crossing
Intraclonal mating in Trypanosoma brucei is associated with out-crossing
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DOI：
10.1099/00221287-143-3-909
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发表时间：
1997-03-01
期刊：
影响因子：
--
通讯作者：
Bailey, M
中科院分区：
文献类型：
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作者：
Gibson, W;Winters, K;Bailey, M
为了检查交配是否可以在布氏锥虫的克隆内以及克隆之间发生，我们用赋予对潮霉素或遗传霉素的抗性的异源基因转化了三个布氏锥虫亚种原种，并在所有可能的双药物组合中进行了一系列的克隆间和克隆内交配。从六个异交中的三个中回收了双药物抗性杂交体，但从三个克隆内交配中的任何一个中都没有回收到双药物抗性杂交体，然而，进一步分析克隆后代锥虫从一个异交使用RFLP标记，分子核型分析和RAPD（多态性DNA的随机扩增）产生了明确的证据，表明已经发生了克隆内以及克隆间交配。克隆间交配的后代是双重耐药的，并且在所检查的13个位点中的9个位点上是杂合的。克隆内交配的后代没有来自潮霉素抗性亲本的可证实的遗传物质输入，并且对于大多数标记而言与遗传霉素抗性亲本相似，除了在遗传霉素抗性亲本中杂合但在这些克隆中纯合的5个位点之外此外，PFGE显示这些克隆中有相当多的核型重排，RAPD和VSG显示遗传物质的丢失是明显的（变异表面糖蛋白）基因指纹分析，我们得出结论，在锥虫中可以发生克隆内交配，但仅在异交期间，这表明减数分裂和/或融合是由可扩散因子触发的。
To examine whether mating can occur within as well as between clones of Trypanosoma brucei, we transformed three T, brucei subspecies stocks with heterologous genes conferring resistance to either hygromycin or Geneticin and carried out a series of inter- and intraclone matings in all possible double drug combinations, Double drug-resistant hybrids were recovered from three of the six out-crosses, but not from any of the three intraclone matings, However, further analysis of cloned progeny trypanosomes from one of the out-crosses using RFLP markers, molecular karyotyping and RAPD (random amplification of polymorphic DNA) produced unequivocal evidence that intra- as well as interclone mating had occurred, The progeny of interclone mating were double drug-resistant and heterozygous at 9 of 13 loci examined, In contrast, the progeny of intraclone mating had no demonstrable input of genetic material from the hygromycin-resistant parent and were similar to the Geneticin-resistant parent for most markers, except for five loci which were heterozygous in the Geneticin-resistant parent but homozygous in these clones (aldolase, THT1 glucose transporter, procyclin, tubulin and cDNA 23), In addition, PFGE showed considerable karyotypic rearrangements in these clones and loss of genetic material was evident from RAPD and VSG (variant surface glycoprotein) gene fingerprint analysis, We conclude that intraclone mating can occur in trypanosomes, but only during out-crossing, suggesting that meiosis and/or fusion are triggered by a diffusible factor.