High serum levels of thrombospondin-1 in patients with idiopathic interstitial pneumonia
High serum levels of thrombospondin-1 in patients with idiopathic interstitial pneumonia
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DOI：
10.1016/j.rmed.2008.05.009
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发表时间：
2008-11-01
影响因子：
4.3
通讯作者：
Kohno, Shigeru
中科院分区：
文献类型：
--
作者：
Ide, Mioko;Ishii, Hiroshi;Kohno, Shigeru
血小板spontin -1 （TSP-1）是一种多功能的基质糖蛋白，可以激活转化生长因子- β，转化生长因子是一种重要的促纤维化细胞因子，参与多种纤维化疾病。TSP-1在博莱霉素诱导的肺纤维化动物模型和一些间质性肺疾病患者的肺组织中表达。本研究探讨了特发性间质性肺炎（IIPs）患者血清和支气管肺泡灌洗液（BALF）中TSP-1的水平及其与其他临床指标的关系。的因素。对病理诊断为IIPs的45例患者（22例为常见于间质性肺炎（UIP）， 23例为非特异性间质性肺炎）进行血清TSP-1及BALF检测。肺炎（NSIP）]，以及28例肺结节病患者和15名健康志愿者使用竞争性酶免疫测定。免疫组化染色分析肺组织中TSP-1的表达和定位。IIPs患者血清TSP-1水平明显高于结节病患者或对照组。这些水平与血管生成细胞因子血管内皮生长因子的水平密切相关，而与%VC呈负相关。再生肺泡上皮和肺泡巨噬细胞中主要可见TSP-1阳性免疫染色。我们的研究结果表明，循环TSP-1水平与间质性肺炎的存在有关，但在我们确定TSP-1在这些疾病的发病机制中起作用之前，还需要进一步的研究。(C) 2008 Elsevier Ltd版权所有。
Thrombospondin-1 (TSP-1), a multifunctional matricellular glycoprotein, can activate transforming growth factor-beta, an important profibrotic cytokine involved in various fibrotic diseases. TSP-1 is expressed in the lung tissue of animal models of bleomycin-induced pulmonary fibrosis and in patients with some interstitial lung diseases. The present study investigated the serum and bronchoalveolar lavage fluid (BALF) levels of TSP-1 in the idiopathic interstitial pneumonias (IIPs) and the relationship between these levels and other clinical. factors. The TSP-1 in the serum and BALF were measured in 45 patients with pathologically diagnosed IIPs [22 with usual interstitial pneumonia (UIP), 23 patients with non-specific interstitial. pneumonia (NSIP)], as well as in 28 patients with pulmonary sarcoidosis and 15 healthy volunteers using a competitive enzyme immunoassay. The expression and localization of TSP-1 in the lungs were analyzed by immunohistochemical staining. The serum TSP-1 levels were significantly higher in patients with IIPs than in either those with sarcoidosis or the controls. These levels correlated well with those of an angiogenic cytokine vascular endothelial growth factor while correlating inversely with the %VC. Positive immunostaining of TSP-1 was predominantly observed in the regenerated alveolar epithelium and alveolar macrophages in the lung. Our findings suggest that the circulating TSP-1 levels are associated with the presence of interstitial pneumonia, but further studies are required before we can definitively conclude that TSP-1 plays a role in the pathogenesis of these diseases. (C) 2008 Elsevier Ltd. All rights reserved.