Sex-specific effects of relaxin-3 on food intake and brain expression of corticotropin-releasing factor in rats.
Sex-specific effects of relaxin-3 on food intake and brain expression of corticotropin-releasing factor in rats.
复制标题
DOI：
10.1210/en.2014-1743
复制
发表时间：
2015-02
期刊：
影响因子：
4.8
通讯作者：
Christophe Lenglos;J. Calvez;E. Timofeeva
中科院分区：
文献类型：
--
作者：
Christophe Lenglos;J. Calvez;E. Timofeeva
本研究比较了松弛素-3(RLN3)对雄性和雌性大鼠摄食量、血浆皮质酮和促肾上腺皮质激素释放因子(CRF)表达的影响。侧脑室注射不同浓度的RLN3，浓度分别为25、200和800pmoL。在注射后30分钟和60分钟，RLN3增加雌性大鼠的摄食量(克)，但雄性大鼠不增加。雌性大鼠在30分钟时所有RLN3浓度的摄食量(mg/gBW)和在60分钟时RLN3的800pmolRLN3的摄入量相对于体重(BW)的增加也更大。此外，800pmolRLN3显著增加雌性大鼠的24小时体重增加，但对雄性大鼠无明显影响。给药60min后，800pmoRLN3引起雄性大鼠血浆皮质酮水平显著升高，下丘脑室旁核(PVN)CRF和c-fos mRNAs表达显著增加，而雌性大鼠则无明显变化。RLN3组大细胞室旁核c-fos基因表达增加，但性别差异无统计学意义。相反，雌性大鼠视前内侧区CRF mRNA的表达增加，但对800pmolRLN3不敏感。在终纹床核，800pmolRLN3可显著增加雌性大鼠CRF基因的表达，但对雄性大鼠无明显影响。因此，雌性大鼠对RLN3表现出更高的敏感性和更强的摄食量增加。RLN3对终纹PVN和床核CRF表达的不同影响可能是导致行为反应性别差异的原因之一。
This study compared the effects of relaxin-3 (RLN3) on food intake, plasma corticosterone, and the expression of corticotropin-releasing factor (CRF) in male and female rats. RLN3 was injected into the lateral ventricle at 25, 200, and 800 pmol concentrations. RLN3 at 25 pmol increased food intake (grams) at 30 and 60 minutes after injection in female but not male rats. Female rats also showed higher increase in relative to body weight (BW) food intake (mg/g BW) for all RLN3 concentrations at 30 minutes and for 800 pmol of RLN3 at 60 minutes. Moreover, RLN3 at 800 pmol significantly increased 24-hour BW gain in female but not male rats. At 60 minutes after administration, 800 pmol of RLN3 produced a significant increase in plasma corticosterone and in the expression of CRF and c-fos mRNAs in the parvocellular paraventricular hypothalamic nucleus (PVN) in male but not female rats. The levels of c-fos mRNA in the magnocellular PVN were increased by RLN3 but did not differ between the sexes. Conversely, expression of CRF mRNA in the medial preoptic area was increased in female rats but was not sensitive to 800 pmol of RLN3. In the bed nucleus of the stria terminalis, 800 pmol of RLN3 significantly increased CRF mRNA expression in female but not male rats. Therefore, female rats showed more sensitivity and stronger food intake increase in response to RLN3. The differential effects of RLN3 on CRF expression in the PVN and bed nucleus of the stria terminalis may contribute to the sex-specific difference in the behavioral response.