Enrichment of liposomal nanomedicines using monolithic solid phase extraction discs following preactivation with bivalent metal ion solutions
Enrichment of liposomal nanomedicines using monolithic solid phase extraction discs following preactivation with bivalent metal ion solutions
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使用二价金属离子溶液预活化后的整体固相萃取盘富集脂质体纳米药物
DOI：
10.1016/j.chroma.2018.06.011
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发表时间：
2018
影响因子：
4.1
通讯作者：
Kato Masaru
中科院分区：
文献类型：
--
作者：
Watanabe Ayako;Karasawa Koji;Murayama Shuhei;Sano Yoshihiro;Takagi Mio;Yamamoto Eiichi;Murata Shigeo;Kato Masaru
硅酸盐是一种优良的吸附剂，因为它的表面积大，易于表面改性。在这项研究中，代表性的脂质体纳米药物DOXIL®和AmBisome®使用直径4.2 mm，长度1.5 mm具有双峰孔的硅胶整体盘富集。虽然当水作为预活化溶液时，纳米颗粒通过圆盘而没有保留，但当使用1 M二价金属（如Mg2+， Ca2+和Ni2+）溶液时，它们被圆盘强烈保留。值得注意的是，聚乙二醇化脂质体纳米颗粒DOXIL与5 μm和10 nm孔组成的圆盘具有较强的亲和性，在40倍稀释的溶液中，DOXIL的回收率接近100%。综上所述，结果表明单片圆盘对于纳米药物脂质体的富集是有效的。
Silicate is an excellent adsorbent because of its large surface area and amenability to surface modification. In this study, the representative liposome nanomedicines DOXIL®and AmBisome®were enriched using a silica monolith disc (diameter 4.2 mm, length 1.5 mm) with bimodal pores. Although the nanoparticles passed through the disc without retention when water was used as the preactivation solution, they were strongly retained by the disc when a 1 M bivalent metal (such as Mg2+, Ca2+, and Ni2+) solution was used. Notably, strong affinity was observed to DOXIL, a pegylated liposomal nanoparticle, by the disc composed of 5 μm and 10 nm through- and meso pores, respectively, and nearly 100% of DOXIL was recovered from a 40× diluted solution. Overall, the results demonstrate that monolithic discs are effective for the enrichment of liposomal nanomedicines.