Ultrastructure of laticifers in redifferentiated organs on callus from Papaver somniferum (Papaveraceae)

Ultrastructure of laticifers in redifferentiated organs on callus from Papaver somniferum (Papaveraceae)
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罂粟愈伤组织再分化器官中乳管的超微结构

DOI:
10.1139/b79-086
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发表时间:
1979
期刊:
影响因子:
--
通讯作者:
P. Mahlberg
P. Mahlberg
中科院分区:
--
文献类型:
--
作者:
C. Nessler;P. Mahlberg

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愈伤组织从罂粟的幼苗下胚轴和成熟茎开始并在培养物中维持。愈伤组织细胞的超微结构类似于完整植物的薄壁组织,其最显着的特征是大的中央液泡。在转移至适当培养基的愈伤组织表面上随机形成分生组织。在横截面中,分生组织表现为被几层同心排列的细胞包围的内部分生组织区域。与愈伤组织相比,分生组织的细胞更小,细胞质更致密,排列更紧凑。由愈伤组织诱导形成的根和芽具有在形态和发育上与完整植物中的乳管相似的细胞。再分化器官中的乳管,与完整植物中的乳管一样,含有许多带帽的囊泡,这些囊泡显然源自堆叠的内质网。再分化器官中相邻乳管元件之间的细胞壁穿孔是由逐渐的、s...
Callus was initiated from seedling hypocotyls and mature stems of Papaver somniferum and maintained in culture. The ultrastructure of callus cells resembled parenchyma of intact plants, their most conspicuous feature being a large central vacuole. Meristemoids were formed at random on the surface of calli transferred to the appropriate medium. In cross section, meristemoids appeared as an inner meristematic region surrounded by several layers of concentrically arranged cells. Cells of meristemoids were smaller, more densely cytoplasmic, and more compactly arranged compared with callus. Roots and shoots induced to form from callus possessed cells that were morphologically and developmentally similar to laticifers in intact plants. Laticifers in redifferentiated organs, like those in intact plants, contained numerous capped vesicles that are apparently derived from stacked endoplasmic reticulum. Cell wall perforations between adjacent laticifer elements in redifferentiated organs developed by the gradual, s...