Staphylococcus aureus fibronectin binding proteins are essential for internalization by osteoblasts but do not account for differences in intracellular levels of bacteria
Staphylococcus aureus fibronectin binding proteins are essential for internalization by osteoblasts but do not account for differences in intracellular levels of bacteria
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DOI：
10.1128/iai.69.5.2872-2877.2001
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发表时间：
2001-05-01
影响因子：
3.1
通讯作者：
Nair, SP
中科院分区：
文献类型：
--
作者：
Ahmed, S;Meghji, S;Nair, SP
金黄色葡萄球菌是骨的主要病原体，已被证明可通过受体介导的途径被成骨细胞内化。在这里，我们报告说，成骨细胞对金黄色葡萄球菌的摄取存在菌株依赖性差异。金黄色葡萄球菌败血性关节炎分离株LS-1的内化程度比实验室菌株8325-4多10倍。这两种金黄色葡萄球菌菌株中纤连蛋白结合蛋白基因的破坏阻止了它们被成骨细胞内化的能力，从而证明了这些基因在此过程中的重要性。然而，这两种菌株与纤连蛋白或成骨细胞结合的能力没有差异，对两种菌株内化动力学的分析如下：成骨细胞显示，菌株8325-4仅在短时间内（2小时）内化且数量较少，而LS-1被成骨细胞大量摄取超过3小时，这些动力学差异 摄取解释了金黄色葡萄球菌的两种菌株在不同程度上被成骨细胞内化的事实，并表明除了纤连蛋白结合蛋白之外，还有其他未确定的毒力因子在内化过程中发挥作用。
Staphylococcus aureus is a major pathogen of bone that has been shown to be internalized by osteoblasts via a receptor-mediated pathway. Here we report that there are strain-dependent differences in the uptake of S. aureus by osteoblasts. An S, aureus septic arthritis isolate, LS-l, was internalized some IO-fold more than the laboratory strain 8325-4. Disruption of the genes for the fibronectin binding proteins in these two strains of S, aureus blocked their ability to be internalized by osteoblasts, thereby demonstrating the essentiality of these genes in this process. However, there were no differences in the capacity of these two strains to bind to fibronectin or osteoblasts, Analysis of the kinetics of internalization of the two strains by: osteoblasts revealed that strain 8325-4 was internalized only over a short period of time (2 h) and to low numbers, while LS-1 was taken up by osteoblasts in large numbers for over 3 h, These differences in the kinetics of uptake explain the fact that the two strains of S, aureus are internalized by osteoblasts to different extents and suggest that in addition to the fibronectin binding proteins there are other, as Set undetermined virulence factors that play a role in the internalization process.