Human placenta-derived cells have mesenchymal stem/progenitor cell potential
Human placenta-derived cells have mesenchymal stem/progenitor cell potential
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DOI：
10.1634/stemcells.22-5-649
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发表时间：
2004-01-01
期刊：
影响因子：
5.2
通讯作者：
Tsuji, K
中科院分区：
文献类型：
--
作者：
Fukuchi, Y;Nakajima, H;Tsuji, K
间充质干细胞广泛分布于成人体内的多种组织(如骨髓、肾、肺、肝等)。这些细胞也存在于胎儿环境中(例如，血液、肝脏、骨髓和肾脏)。然而，在这些组织中，间充质干细胞是一个罕见的群体。在这里，我们试图在人类胎盘中鉴定出具有MSC样活性的细胞。我们从胰酶消化的足月胎盘中分离出贴壁细胞，并通过限制稀释建立了两个克隆。我们检测了这些细胞的形态、表面标记、基因表达模式和分化潜能，发现它们表达几种干细胞标记、造血/内皮细胞相关基因和器官特异性基因，通过逆转录-聚合酶链式反应和荧光激活细胞分选分析确定。在适当的条件下，它们还显示出成骨和成脂分化潜能。我们认为胎盘来源的细胞在形态、细胞表面抗原表达和基因表达模式方面具有与MSCs相似的多系分化潜能。胎盘可能被证明是间充质干细胞的有用来源。
Mesenchymal stem/progenitor cells (MSCs) are widely distributed in a variety of tissues in the adult human body (e.g., bone marrow [BM], kidney, lung, and liver). These cells are also present in the fetal environment (e.g., blood, liver, BM, and kidney). However, MSCs are a rare population in these tissues. Here we tried to identify cells with MSC-like potency in human placenta. We isolated adherent cells from trypsin-digested term placentas and established two clones by limiting dilution. We examined these cells for morphology, surface markers, gene expression patterns, and differentiation potential and found that they expressed several stem cell markers, hematopoietic/endothelial cell-related genes, and organ-specific genes, as determined by reverse transcription-polymerase chain reaction and fluorescence-activated cell sorter analysis. They also showed osteogenic and adipogenic differentiation potentials under appropriate conditions. We suggest that placenta-derived cells have multilineage differentiation potential similar to MSCs in terms of morphology, cell-surface antigen expression, and gene expression patterns. The placenta may prove to be a useful source of MSCs.