Correlation of morphologic and cytogenetic parameters of genetic instability with chromosomal alterations in in situ carcinomas of the breast
Correlation of morphologic and cytogenetic parameters of genetic instability with chromosomal alterations in in situ carcinomas of the breast
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DOI：
10.1309/xcpg-cr87-6u8d-b11k
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发表时间：
2000-12-01
影响因子：
3.5
通讯作者：
Böcker, W
中科院分区：
文献类型：
--
作者：
Buerger, H;Mommers, EC;Böcker, W
使用生物标记物可以更好地建立浸润前乳腺疾病的分类，从而提高可重复性。我们通过比较基因组杂交研究了57例乳腺导管和小叶原位癌，并将这些发现与定量特征如平均核面积、有丝分裂指数（MI）、凋亡指数（AI）以及有无坏死相关联。8p的丢失以及8q和6q的增加分别与显著较高的MI和AI相关，而16 q缺失与MI和AI降低相关。每例病例中，在6q、8q和17q增加的肿瘤和13q丢失的肿瘤中观察到显著更高数量的改变。16 q的丢失和17 q的增加分别与坏死的存在或不存在相关。我们的数据清楚地表明，不同的细胞遗传学变化与表型变化，增殖和凋亡。这些数据可用于改进现有的分类方案。
Classification of preinvasive breast disease could be better founded using biologic markers, thereby increasing reproducibility. We studied 57 breast ductal and lobular in situ carcinomas by means of comparative genomic hybridization and correlated these findings with quantitative features such as the mean nuclear area, mitotic index (MI), apoptotic index (AI), and the presence or absence of necrosis.Loss of 8p and gains of 8q and 6q were associated, respectively, with a significantly higher MI and AI, whereas loss of 16q was associated with a lower MI and AI. A significantly higher number of alterations per case were seen in tumors I-vith gains of 6q, 8q, and 17q and tumors with loss of 13q. Loss of 16q and gain of 17q correlated with the absence or presence of necrosis, respectively. Our data clearly demonstrate that distinct cytogenetic changes correlate with phenotypic changes, proliferation, and apoptosis. These data may be used to refine existing classification schemes.