PetC1 Is the Major Rieske Iron-Sulfur Protein in the Cytochrome b6f Complex of Synechocystis sp. PCC 6803*
PetC1 Is the Major Rieske Iron-Sulfur Protein in the Cytochrome b6f Complex of Synechocystis sp. PCC 6803*
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PetC1 是集胞藻细胞色素 b6f 复合物中的主要 Rieske 铁硫蛋白。
DOI：
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发表时间：
2004
影响因子：
4.8
通讯作者：
M. Rögner
中科院分区：
文献类型：
--
作者：
D. Schneider;S. Berry;T. Volkmer;A. Seidler;M. Rögner
许多完全测序的蓝藻基因组包含一个基因家族，编码可能的Rieske铁硫蛋白。Rieske蛋白是参与呼吸和光合作用电子传递的细胞色素BC型复合体的大亚基之一。与由单基因编码的该复合体的所有其他亚基不同，聚球藻PCC6803的基因组包含三个PETC基因，所有这些基因都编码潜在的Rieske亚基。最有趣的是，任何PETC基因都可以单独删除，而不会显著改变集胞藻的表型。相反，双缺失实验表明，petC1和petC2不能同时缺失，而petC3可以与其他两个PETC基因中的任何一个一起缺失。进一步的结果表明，每种Rieske蛋白具有不同的生理功能。而PetC2可以部分取代主要的Rieske异构体PetC1，而PetC3不能在功能上取代PetC1或PetC2，并且可能具有特殊的功能，涉及到氧化还原电势低于质体对苯二酚的特殊给体。突变分析和对突变菌株中电子转移反应的详细描述表明，与PetC2不同的是，PetC1起主导作用。
Many of the completely sequenced cyanobacterial genomes contain a gene family that encodes for putative Rieske iron-sulfur proteins. The Rieske protein is one of the large subunits of the cytochrome bc-type complexes involved in respiratory and photosynthetic electron transfer. In contrast to all other subunits of this complex that are encoded by single genes, the genome of the cyanobacterium Synechocystis PCC 6803 contains three petC genes, all encoding potential Rieske subunits. Most interestingly, any of the petC genes can be deleted individually without altering the Synechocystis phenotype dramatically. In contrast, double deletion experiments revealed that petC1 and petC2 cannot be deleted in combination, whereas petC3 can be deleted together with any of the other two petC genes. Further results suggest a different physiological function for each of the Rieske proteins. Whereas PetC2 can partly replace the dominating Rieske isoform PetC1, PetC3 is unable to functionally replace either PetC1 or PetC2 and may have a special function involving a special donor with a lower redox potential than plastoquinone. A predominant role of PetC1, which is (partly) different from PetC2, is suggested by the mutational analysis and a detailed characterization of the electron transfer reactions in the mutant strains.
DOI：
10.1073/pnas.97.9.4567
发表时间：
2000-04-25
影响因子：
11.1
作者：
Darrouzet, E;Valkova-Valchanova, M;Daldal, F
通讯作者：
Daldal, F
Rieske 蛋白柔性接头区域长度的变化会损害泛醇与细胞色素 bc1 复合物的相互作用。
DOI：
10.1046/j.1432-1327.2000.01650.x
发表时间：
2000
期刊：
European journal of biochemistry
影响因子：
--
作者：
Nett,JH;Hunte,C;Trumpower,BL
通讯作者：
Trumpower,BL
在细胞色素 bc1 复合物铁硫蛋白的系链区域用亮氨酸取代丙氨酸 86 会影响 [2Fe2S] 结构域的迁移率。
影响因子：
2.9
作者：
Ghosh,M;Wang,Y;Ebert,CE;Vadlamuri,S;Beattie,DS
通讯作者：
Beattie,DS