Immunoglobulin and T-cell receptor beta-chain gene rearrangement analysis of Hodgkin's disease: implications for lineage determination and differential diagnosis.
Immunoglobulin and T-cell receptor beta-chain gene rearrangement analysis of Hodgkin's disease: implications for lineage determination and differential diagnosis.
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霍奇金病的免疫球蛋白和 T 细胞受体 β 链基因重排分析：对谱系测定和鉴别诊断的影响。
DOI：
10.1073/pnas.83.20.7942
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发表时间：
1986
影响因子：
11.1
通讯作者：
Dalla-Favera,R
中科院分区：
文献类型：
--
作者：
Knowles2nd,DM;Neri,A;Pelicci,PG;Burke,JS;Wu,A;Winberg,CD;Sheibani,K;Dalla-Favera,R
通过分析18例何杰金氏病（HD）免疫球蛋白和T细胞受体β链（T β）基因位点的组织结构，并与103例B和T细胞非何杰金氏淋巴瘤（NHL）和淋巴样白血病（LL）进行比较，研究了HD的谱系和克隆性。在所有103例NHL和LL中，通过免疫基因型分析可容易地检测到相当大的克隆B或T细胞群，占病理样本的10%以上，但在18例HD病例中均未检测到。然而，在18例HD病例中的3例中可检测到极少量的克隆群体（小于或等于1%）。我们证明，这些少数克隆群体不对应于Reed-Sternberg（RS）细胞，因为在HD含有大于25% RS细胞的情况下，无法检测到克隆免疫球蛋白或T β基因重排。这些样品中存在的RS细胞的数量似乎与多克隆T细胞的基因重排特征的模式直接相关。这些研究表明，克隆免疫球蛋白和T β基因重排的Southern印迹杂交分析是HD和NHL鉴别诊断的一种准确、客观的工具; HD主要由多克隆B和T细胞组成; HD中偶尔可以发现与RS细胞无关的少量克隆B或T细胞群; RS细胞不代表克隆B或T细胞扩增。最后，我们的数据初步表明，RS细胞可能代表多克隆T细胞群。
The lineage and clonality of Hodgkin's disease (HD) were investigated by analyzing the organization of the immunoglobulin and T-cell receptor beta-chain (T beta) gene loci in 18 cases of HD, and for comparison, in a panel of 103 cases of B- and T-cell non-Hodgkin's lymphomas (NHLs) and lymphoid leukemias (LLs). Sizable clonal B- or T-cell populations, representing greater than or equal to 10% of the pathologic sample, were readily detectable by immunogenotypic analysis in all 103 NHLs and LLs but not in any of the 18 cases of HD. However, extremely minor clonal populations (less than or equal to 1%) were detectable in 3 of 18 cases of HD. We demonstrated that these minor clonal populations do not correspond to Reed-Sternberg (RS) cells since clonal immunoglobulin or T beta gene rearrangements are not detectable in cases of HD containing greater than 25% RS cells. The number of RS cells present in these samples appeared to correlate directly with the pattern of gene rearrangements characteristic of polyclonal T cells. These studies demonstrate that Southern blot hybridization analysis for clonal immunoglobulin and T beta gene rearrangements represents an accurate, objective tool in the differential diagnosis between HD and NHL; that HD is predominantly composed of polyclonal B and T cells; that minor clonal B- or T-cell populations unrelated to RS cells occasionally can be found in HD; and that RS cells do not represent clonal B- or T-cell expansions. Finally, our data preliminarily suggest that RS cells may represent polyclonal T-cell populations.