Nanoscale analysis of unstained biological specimens in water without radiation damage using high-resolution frequency transmission electric-field system based on FE-SEM
Nanoscale analysis of unstained biological specimens in water without radiation damage using high-resolution frequency transmission electric-field system based on FE-SEM
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DOI：
10.1016/j.bbrc.2015.02.140
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发表时间：
2015-04-10
影响因子：
3.1
通讯作者：
Ogura, Toshihiko
中科院分区：
文献类型：
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作者：
Ogura, Toshihiko
扫描电子显微镜（SEM）已被广泛用于检查细菌，病毒和蛋白质的生物样品。到目前为止，大气和/或潮湿的生物标本已被检查使用各种大气持有人或特殊设备，包括扫描电镜。不幸的是，它们受到直接电子束的严重辐射损伤。此外，水中未染色的生物样品的图像产生差的对比度。我们最近开发了一种新的分析技术，包括基于电子扫描电镜的频率传输电场（FTE）方法。该方法适用于未染色生物样本的高对比度成像。我们的目标是优化方法。在这里，我们描述了一种基于场发射SEM的高分辨率FTE系统，它允许对水中的各种生物标本进行成像和纳米级检查，而不会造成辐射损伤。空间分辨率为8 nm，高于现有FTE系统的41 nm。我们的新方法可以很容易地用于检查未染色的生物标本，包括细菌，病毒和蛋白质复合物。此外，我们的高分辨率FTE系统可用于广泛科学领域的各种液体样品，例如纳米颗粒、纳米管以及有机和催化材料。(C)2015作者爱思唯尔公司出版
Scanning electron microscopy (SEM) has been widely used to examine biological specimens of bacteria, viruses and proteins. Until now, atmospheric and/or wet biological specimens have been examined using various atmospheric holders or special equipment involving SEM. Unfortunately, they undergo heavy radiation damage by the direct electron beam. In addition, images of unstained biological samples in water yield poor contrast. We recently developed a new analytical technology involving a frequency transmission electric-field (FTE) method based on thermionic SEM. This method is suitable for high-contrast imaging of unstained biological specimens. Our aim was to optimise the method. Here we describe a high-resolution FTE system based on field-emission SEM; it allows for imaging and nanoscale examination of various biological specimens in water without radiation damage. The spatial resolution is 8 nm, which is higher than 41 nm of the existing FTE system. Our new method can be easily utilised for examination of unstained biological specimens including bacteria, viruses and protein complexes. Furthermore, our high-resolution FTE system can be used for diverse liquid samples across a broad range of scientific fields, e.g. nanoparticles, nanotubes and organic and catalytic materials. (C) 2015 The Author. Published by Elsevier Inc.