Characterization of intranuclear capsids made by ts morphogenic mutants of HSV-1.
Characterization of intranuclear capsids made by ts morphogenic mutants of HSV-1.
复制标题
HSV-1 ts 形态发生突变体产生的核内衣壳的表征。
DOI：
10.1016/0042-6822(88)90288-7
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发表时间：
1988
期刊：
影响因子：
3.7
通讯作者：
Bachenheimer,SL
中科院分区：
文献类型：
--
作者：
Sherman,G;Bachenheimer,SL
我们已经鉴定了七个温度敏感型HSV-1突变体制造的衣壳，这些突变体在非允许温度（NPT）下被证明在病毒DNA加工和包装中存在缺陷。在NPT中从突变感染细胞中分离出的空衣壳缺乏DNA，与wt B衣壳共同沉积在蔗糖中，并且含有与wt B衣壳中发现的相同的结构蛋白（W. Gibson和B. Roizman(1972)）。Virol.10, 1044 - 1052)。VP22a在空衣壳中的存在表明，这种蛋白从高分子量前体加工及其与衣壳的结合是DNA衣壳化所必需的，但不是充分的。突变体在NPT条件下没有产生可检测到的A衣壳，但在允许温度（PT）下可以产生，这表明A粒子是在包壳过程中或之后产生的，而不是在包壳之前产生的。在温度转移实验中，研究人员发现，当细胞随后转移到PT时，在NPT制备的突变体F18的衣壳没有参与DNA的衣壳化。只有在温度转移到PT后制备的衣壳获得了病毒DNA，这意味着F18的突变可能存在于编码结构蛋白的基因中，或者在参与病毒DNA加工的蛋白质中。
We have characterized capsids made by seven temperature-sensitive (ts) mutants of HSV-1 previously shown to be defective in viral DNA processing and packaging at the nonpermissive temperature (NPT). The empty capsids isolated from mutant-infected cells at the NPT were devoid of DNA, cosedimented in sucrose with wt B capsids, and contained the same structural proteins found in wt B capsids (W. Gibson and B. Roizman (1972).J. Virol.10, 1044–1052). The presence of VP22a in empty capsids suggests that the processing of this protein from higher-molecular-weight precursors and its association with capsids is required, but not sufficient, for DNA encapsidation. Mutants made no detectable A capsids at the NPT, but did so at the permissive temperature (PT), suggesting that A particles are generated during or subsequent to, rather than prior to, encapsidation. In temperature-shift experiments, it was demonstrated that capsids of one of the mutants, F18, made at the NPT did not participate in DNA encapsidation when cells were subsequently shifted to the PT. Only those capsids made after temperature shift to the PT acquired viral DNA, implying that the is mutation in F18 may lie in a gene coding for a structural protein, or in a protein involved in the processing of viral DNA.