Reaction of Human Cd7metallothionein and N-Ethylmaleimide: Kinetic and Structural Insights from Electrospray Ionization Mass Spectrometry
Reaction of Human Cd7metallothionein and N-Ethylmaleimide: Kinetic and Structural Insights from Electrospray Ionization Mass Spectrometry
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DOI：
10.1021/acs.biochem.5b00545
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发表时间：
2015-10-06
期刊：
影响因子：
2.9
通讯作者：
Russell, David H.
中科院分区：
文献类型：
--
作者：
Chen, Shu-Hua;Russell, David H.
用电喷雾电离-离子迁移率-质谱仪(ESI-IM-MS)研究了镉结合的人金属硫蛋白-2A(Cd7MT)与N-乙基马来酰亚胺(NEM)的反应。MS提供了反应进行过程中动力学中间体分布的直接测量，并提供了对Cd7MT中单个金属硫代键的相对动力学稳定性的新见解。各种保留金属的中间体(Cd-I，附着有Cd~(2+)离子的中间体)的速率常数相差3个数量级：Cd-4
The reaction of cadmium-binding human metallothionein-2A (Cd7MT) and N-ethylmaleimide (NEM) is investigated by electrospray ionization-ion mobility-mass spectrometry (ESI IM-MS). MS provides a direct measure of the distribution of the kinetic intermediates as the reaction proceeds and provides new insights into the relative kinetic stability of the individual metal-thiolate bonds in Cd7MT. The rate constants for the various metal-retaining intermediates (Cd-i, intermediate with i Cd2+ ions attached) differ by >3 orders of magnitude: Cd-4