A new method to quantify phagocytosis and intracellular degradation using green fluorescent protein-labeled Escherichia coli:: Comparison of cord blood macrophages and peripheral blood macrophages of healthy adults
A new method to quantify phagocytosis and intracellular degradation using green fluorescent protein-labeled Escherichia coli:: Comparison of cord blood macrophages and peripheral blood macrophages of healthy adults
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DOI：
10.1002/cyto.a.20222
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发表时间：
2006-03-01
期刊：
影响因子：
3.7
通讯作者：
Orlikowsky, T
中科院分区：
文献类型：
--
作者：
Gille, C;Spring, B;Orlikowsky, T
新生儿巨噬细胞（M Phi）中先天性和适应性免疫功能之间的相互作用仍不清楚。因此，我们建立了一种方法来量化细菌的吞噬作用和细胞内降解，使用绿色荧光蛋白（GFP）标记的大肠杆菌结合表型分析。吞噬M Phi的比例，吞噬细菌每M Phi，和细菌降解的动力学是相当的脐带血M Phi的足月新生儿和M Phi的健康成人。表型分析显示CD14和CD16在几分钟内下调。GFP标记的E. coli可能是进一步研究脐带血M Phi亚群和吞噬作用决定因素的有用工具。(c)2006年国际分析细胞学学会。
Interactions between innate and adaptive immune functions in neonatal macrophages (M Phi) are still unclear. We therefore established a method to quantify bacterial phagocytosis and intracellular degradation, using green fluorescent protein (GFP)-labeled Escherichia coli in combination with phenotypic analysis. The kinetics of the proportion of phagocyting M Phi, phagocytosed bacteria per M Phi, and bacterial degradation were comparable for cord blood M Phi of term neonates and M Phi of healthy adults. Phenotyping revealed CD14 and CD16 to be down-modulated within minutes. GFP-labeted E. coli may be useful tools to further study M Phi subpopulations and determinants of phagocytosis in cord blood M Phi. (c) 2006 International Society for Analytical Cytology.