Expression of nerve growth factor in itchy skins of atopic NC/NgaTnd mice
Expression of nerve growth factor in itchy skins of atopic NC/NgaTnd mice
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DOI：
10.1292/jvms.67.915
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发表时间：
2005-09-01
影响因子：
1.2
通讯作者：
Matsuda, H
中科院分区：
文献类型：
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作者：
Tanaka, A;Matsuda, H
虽然神经营养因子可能参与皮肤瘙痒的特应性皮炎已被预测，痒是由诱导的确切机制尚不清楚。由于神经生长因子（NGF）对感觉神经的发育和功能起着至关重要的作用，我们测定了患有或不患有特应性皮炎的NC/ ngatand小鼠皮肤中NGF的产生和神经纤维的延伸。NC/ ngatand小鼠在空气不调节的常规环境中长大时，会自发地发生特应性皮炎样皮肤病变。我们使用一种新的分析系统量化了NC/ ngatand小鼠在皮炎发展过程中的抓挠行为，并与临床皮肤严重程度评分进行了比较。皮炎的严重程度与抓伤次数的增加之间存在显著相关性，表明抓伤行为可能与临床皮肤状况有关。常规NC/ ngatand小鼠皮损中NGF含量显著高于SPF小鼠。NGF在NC/ ngatand小鼠皮肤的角质形成细胞和成纤维细胞中呈阳性反应。免疫组化分析显示，蛋白基因产物9.5阳性神经纤维从真皮向皮损处的表皮延伸。这些结果表明，由NGF诱导的感觉神经可能有助于瘙痒的发展，并且在受影响部位产生的NGF可能导致特应性皮炎的异常皮肤敏感性。
Although the possible involvement of neurotrophic factors in itchy skins of atopic dermatitis has been predicted, the exact mechanism by which itch is induced remains unclear. Since nerve growth factor (NGF) has crucial effects on development and functions of sensory nerves, we determined production of NGF and extension of nerve fibers in skins of NC/NgaTnd mice with or without atopic dermatitis. NC/NgaTnd mice spontaneously develop atopic dermatitis-like skin lesions when they are raised in air-unregulated conventional circumstances. We quantified scratching behavior of NC/NgaTnd mice during the development of dermatitis using a novel analytical system and compared to clinical skin severity scores. A significant correlation between the severity of dermatitis and the increase in the number of scratches was identified, indicating that scratching behavior may associate with clinical skin conditions. NGF contents in the skin lesions of conventional NC/NgaTnd mice were significantly higher than those in SPF mice. Positive reactions for NGF were observed in keratinocytes and fibroblasts in affected skins of conventional NC/NgaTnd mice. Immumohistochemical analysis showed the extension of protein gene product 9.5-positive nerve fibers from the dermis toward the epidermis at the skin lesions. These results suggest that sensory nerves induced by NGF may contribute to development of itch, and that NGF produced at the affected site may provide abnormal skin sensitivity in atopic dermatitis.