Multidrug resistance transporters in the olfactory receptor neurons of Xenopus laevis tadpoles
Multidrug resistance transporters in the olfactory receptor neurons of Xenopus laevis tadpoles
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DOI：
10.1113/jphysiol.2002.033175
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发表时间：
2003-01-15
影响因子：
5.5
通讯作者：
Schild, D
中科院分区：
文献类型：
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作者：
Manzini, I;Schild, D
嗅觉受体神经元（ORN）是唯一一类直接暴露于环境的神经元。因此，他们需要处理异生物质和潜在的细胞毒性物质。在这里，我们第一次表明，ORN拥有转运系统，驱逐外源性物质穿过质膜。使用钙黄绿素和钙指示剂染料作为外源性物质，我们证明，ORNs似乎表达多药耐药P-糖蛋白（MDR 1）和多药耐药相关蛋白（MRP）。这赋予了ORN运输大量底物的能力，包括钙指示染料和钙黄绿素穿过其质膜。相反，阻断P-糖蛋白和MRP增加了这些染料的净摄取。
Olfactory receptor neurons (ORNs) are the only class of neurons that is directly exposed to the environment. Therefore, they need to deal with xenobiotic and potentially cytotoxic substances. Here we show for the first time that ORNs possess transporter systems that expel xenobiotics across the plasma membrane. Using calcein and calcium-indicator dyes as xenobiotics, we demonstrate that ORNs appear to express the multidrug resistance P-glycoprotein (MDR1) and multidrug resistance-associated proteins (MRP). This endows ORNs with the ability to transport a large number of substrates including calcium-indicator dyes and calcein across their plasma membranes. Conversely, blocking P-glycoprotein and MRP increases the net uptake of these dyes.