Maternal testosterone and placental function: Effect of electroacupuncture on placental expression of angiogenic markers and fetal growth
Maternal testosterone and placental function: Effect of electroacupuncture on placental expression of angiogenic markers and fetal growth
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DOI：
10.1016/j.mce.2016.05.014
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发表时间：
2016-09-15
影响因子：
4.1
通讯作者：
Stener-Victorin, Elisabet
中科院分区：
文献类型：
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作者：
Fornes, Romina;Hu, Min;Stener-Victorin, Elisabet
多囊卵巢综合征（PCOS）患者在妊娠期间循环雄激素升高，不良妊娠结局的风险增加。在这里，我们测试的假设，母体雄激素过多减少胎盘和胎儿的生长，和胎盘的类固醇生成，血管生成和交感神经活性的标志物的表达，并与低频电刺激针灸防止这些变化。妊娠大鼠在妊娠第15-19天（GD）暴露于溶媒或睾酮。在GD 16 -20对对照或睾酮暴露的母兽给予低频电针（EA）或处理（作为EA程序的对照）。在GD 21，测量血压并采集母体血液、胎仔和胎盘。胎盘类固醇受体的表达和蛋白质参与血管生成，神经营养和肾上腺素能signalings.EA没有影响任何变量在对照组大鼠，除了母体血清皮质酮，这是减少。与对照组相比，在睾酮暴露的母鼠中，EA使收缩压增加了30%，降低了循环去甲肾上腺素和皮质酮、胎儿和胎盘重量以及胎盘VEGFR 1和proNGF蛋白表达，并增加了VEGFA/VEGFR 1比率、成熟神经生长因子（mNGF）和mNGF/proNGF比率。总之，在对照组动物中，低频EA对任何研究变量都没有任何负面影响。与此相反，在怀孕母鼠暴露于睾酮EA增加血压和受损的胎盘生长和功能，导致胎儿生长下降。(C)由Elsevier爱尔兰Ltd.出版
Women with polycystic ovary syndrome (PCOS) have elevated circulating androgens during pregnancy and are at an increased risk of adverse pregnancy outcomes. Here we tested the hypotheses that maternal androgen excess decrease placental and fetal growth, and placental expression of markers of steroidogenesis, angiogenesis and sympathetic activity, and that acupuncture with low-frequency electrical stimulation prevents these changes. Pregnant rats were exposed to vehicle or testosterone on gestational day (GD)15-19. Low-frequency electroacupuncture (EA) or handling, as a control for the EA procedure, was given to control or testosterone exposed dams on GD16-20. On GD21, blood pressure was measured and maternal blood, fetuses and placentas collected. Placental steroid receptor expression and proteins involved in angiogenic, neurotrophic and adrenergic signaling were analyzed.EA did not affect any variables in control rats except maternal serum corticosterone, which was reduced. EA in testosterone exposed dams compared with controls increased systolic pressure by 30%, decreased circulating norepinephrine and corticosterone, fetal and placental weight and placental VEGFR1 and proNGF protein expression, and increased the VEGFA/VEGFR1 ratio, mature NGF (mNGF) and the mNGF/proNGF ratio.In conclusion, low-frequency EA in control animals did not have any negative influence on any of the studied variables. In contrast, EA in pregnant dams exposed to testosterone increased blood pressure and impaired placental growth and function, leading to decreased fetal growth. (C) 2016 Published by Elsevier Ireland Ltd.